PFDN4 recombinant protein

PFDN4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121631

• Gene Names: PFDN4; C1; PFD4
• Purity: >80% as determined by SDS-PAGE
• Synonyms: PFDN4; PFDN4 Protein; Human; Recombinant (His Tag); Human PFDN4/PFD4 Protein (His Tag); C1 Protein; PFD4 Protein; prefoldin subunit 4; PFDN4 recombinant protein

• Host: E Coli
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 5% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser134

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human PFDN4 (Q9NQP4)(Met1-Ser134) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PFDN4 recombinant protein

Background: PFDN4 is a member of the prefoldin beta subunit family. It is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. PFDN4 binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. PFDN4 also binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

References

Iijima M, et al. (1996) Cloning of cDNA with possible transcription factor activity at the G1-S phase transition in human fibroblast cell lines. Acta Med Okayama. 50(2):73-7. Hartl FU, et al. (2002) Molecular chaperones in the cytosol: from nascent chain to folded protein. Science. 295(5561):1852-8. Vainberg I, et al. (1998) Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin. Cell. 93(5):863-73.

NCBI and Uniprot Product Information

• NCBI GI #: 12643815
• NCBI GeneID: 5203
• UniProt Accession #: Q9NQP4; Q5TD11; Q92779
• Molecular Weight: Calculated: 15kDa; Observed: 15kDa
• NCBI Official Full Name: Prefoldin subunit 4
• NCBI Official Synonym Full Names: prefoldin subunit 4
• NCBI Official Symbol: PFDN4
• NCBI Official Synonym Symbols: C1; PFD4
• NCBI Protein Information: prefoldin subunit 4; prefoldin 4; protein C-1
• UniProt Protein Name: Prefoldin subunit 4
• Protein Family: Prefoldin
• UniProt Gene Name: PFDN4
• UniProt Synonym Gene Names: PFD4
• UniProt Entry Name: PFD4_HUMAN

NCBI Description

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq, Jul 2008]

Uniprot Description

PFDN4: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family.

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: mitochondrion; cytoplasm; nucleus; cytosol; prefoldin complex

Molecular Function: protein binding; chaperone binding; unfolded protein binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding