Human Ribonucleoside-diphosphate reductase large subunit ELISA Kit | RRM1 elisa kit

Human Ribonucleoside-diphosphate reductase large subunit ELISA Kit

Cat. Num. AAA760942

• Gene Names: RRM1; R1; RR1; RIR1
• Reactivity: Human
• Synonyms: Ribonucleoside-diphosphate reductase large subunit; Human Ribonucleoside-diphosphate reductase large subunit ELISA Kit; RRM1/R1/RR1/RIR1/Ribonucleoside-diphosphate reductase subunit M1/Ribonucleotide reductase large subunit; RRM1 elisa kit

• Reactivity: Human
• Sequence Length: 792

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RRM1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RRM1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RRM1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RRM1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RRM1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506749
• NCBI GeneID: 6240
• NCBI Accession #: NP_001024.1
• NCBI GenBank Nucleotide #: NM_001033.4
• Molecular Weight: 90,070 Da
• NCBI Official Full Name: ribonucleoside-diphosphate reductase large subunit isoform 1
• NCBI Official Synonym Full Names: ribonucleotide reductase catalytic subunit M1
• NCBI Official Symbol: RRM1
• NCBI Official Synonym Symbols: R1; RR1; RIR1
• NCBI Protein Information: ribonucleoside-diphosphate reductase large subunit
• UniProt Protein Name: Ribonucleoside-diphosphate reductase large subunit
• Protein Family: Ribonucleoside-diphosphate reductase
• UniProt Gene Name: RRM1
• UniProt Synonym Gene Names: RR1
• UniProt Entry Name: RIR1_HUMAN

NCBI Description

This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

RRM1: an enzyme involved in DNA replication that provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Belongs to the ribonucleoside diphosphate reductase large chain family. Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the M1 subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site. Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2. The level of the enzyme activity is closely correlated with the growth rate of a cell and appears to vary with the cell cycle. Patients with advanced non-small cell lung cancer responded favorably to gemcitabine.

Protein type: Oxidoreductase; Nucleotide Metabolism - pyrimidine; Other Amino Acids Metabolism - glutathione; EC 1.17.4.1; DNA replication; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cell projection; cell soma; cytoplasm; cytosol; nuclear envelope; nucleoplasm; ribonucleoside-diphosphate reductase complex

Molecular Function: ATP binding; protein binding; ribonucleoside-diphosphate reductase activity

Biological Process: cell proliferation in forebrain; deoxyribonucleotide biosynthetic process; DNA replication; male gonad development; mitotic cell cycle; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; protein heterotetramerization; pyrimidine base metabolic process; response to ionizing radiation; retina development in camera-type eye