DNA-directed RNA polymerase II subunit RPB2 (RPB2) Recombinant Protein | RPB2 recombinant protein

Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase II subunit RPB2 (RPB2) , partial

• Gene Names: RPB2; RPB150; RPO22; SIT2; SOH2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-directed RNA polymerase II subunit RPB2 (RPB2); Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase II subunit RPB2 (RPB2); partial; RPB2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,224

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 398365417
• NCBI GeneID: 854322
• NCBI Accession #: NP_014794.3
• NCBI GenBank Nucleotide #: NM_001183570.3
• UniProt Accession #: P08518; Q12738; Q7Z9Y0; D6W2K8
• Molecular Weight: 138,751 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II core subunit RPB2
• NCBI Official Symbol: RPB2
• NCBI Official Synonym Symbols: RPB150; RPO22; SIT2; SOH2
• NCBI Protein Information: DNA-directed RNA polymerase II core subunit RPB2
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB2
• UniProt Gene Name: RPB2
• UniProt Synonym Gene Names: RPB150; RPO22; RNA polymerase II subunit 2

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerases II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. During a transcription cycle, Pol II, general transcription factors and the Mediator complex assemble as the preinitiation complex (PIC) at the promoter. 11-15 base pairs of DNA surrounding the transcription start site are melted and the single-stranded DNA template strand of the promoter is positioned deeply within the central active site cleft of Pol II to form the open complex. After synthesis of about 30 bases of RNA, Pol II releases its contacts with the core promoter and the rest of the transcription machinery (promoter clearance) and enters the stage of transcription elongation in which it moves on the template as the transcript elongates. Pol II appears to oscillate between inactive and active conformations at each step of nucleotide addition. Pol II is composed of mobile elements that move relative to each other. The core element with the central large cleft comprises RPB3, RBP10, RPB11, RPB12 and regions of RPB1 and RPB2 forming the active center. The clamp element (portions of RPB1, RPB2 and RPB3) is connected to the core through a set of flexible switches and moves to open and close the cleft. The cleft is surrounded by jaws: an upper jaw formed by portions of RBP1, RPB2 and RPB9, and a lower jaw. The jaws are thought to grab the incoming DNA template. The fork loop 1 (RPB2) interacts with the RNA-DNA hybrid, possibly stabilizing it.MiscellaneousThe binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits.Present with 18700 molecules/cell in log phase SD medium.