Mouse anti-Human PHLDA1 Monoclonal Antibody | anti-PHLDA1 antibody

PHLDA1 (HRIP, TDAG51, Pleckstrin Homology-like Domain Family A Member 1, Apoptosis-associated Nuclear Protein, Proline- and Glutamine-rich Protein, Proline- and Histidine-rich Protein, T-cell Death-associated Gene 51 Protein) (AP)

Cat. Num. AAA6132920

• Gene Names: PHLDA1; PHRIP; TDAG51; DT1P1B11
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PHLDA1; Monoclonal Antibody; PHLDA1 (HRIP; TDAG51; Pleckstrin Homology-like Domain Family A Member 1; Apoptosis-associated Nuclear Protein; Proline- and Glutamine-rich Protein; Proline- and Histidine-rich Protein; T-cell Death-associated Gene 51 Protein) (AP); anti-PHLDA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3A5
• Specificity: Recognizes human PHLDA1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PHLDA1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1-110 from PHLDA1 (NP_031376) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVERKGKYMY*
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (38.1kD).)

Testing Data

(Detection limit for recombinant GST tagged PHLDA1 is 0.3ng/ml as a capture antibody.)

Related Product Information for anti-PHLDA1 antibody

This gene encodes an evolutionarily conserved proline-histidine rich nuclear protein. The encoded protein may play an important role in the anti-apoptotic effects of insulin-like growth factor-1.

Product Categories/Family for anti-PHLDA1 antibody

Antibodies; Pleckstrin Homology Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 83977459
• NCBI GeneID: 22822
• NCBI Accession #: NP_031376
• NCBI GenBank Nucleotide #: NM_007350
• UniProt Accession #: Q8WV24
• Molecular Weight: 45kDa
• NCBI Official Full Name: pleckstrin homology-like domain family A member 1
• NCBI Official Synonym Full Names: pleckstrin homology like domain family A member 1
• NCBI Official Symbol: PHLDA1
• NCBI Official Synonym Symbols: PHRIP; TDAG51; DT1P1B11
• NCBI Protein Information: pleckstrin homology-like domain family A member 1
• UniProt Protein Name: Pleckstrin homology-like domain family A member 1
• Protein Family: Pleckstrin homology-like domain family
• UniProt Gene Name: PHLDA1
• UniProt Synonym Gene Names: PHRIP; TDAG51; PQ-rich protein; PQR protein
• UniProt Entry Name: PHLA1_HUMAN

NCBI Description

This gene encodes an evolutionarily conserved proline-histidine rich nuclear protein. The encoded protein may play an important role in the anti-apoptotic effects of insulin-like growth factor-1. [provided by RefSeq, Jul 2008]

Uniprot Description

PHLDA1: Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.

Protein type: Nucleolus; Apoptosis

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: nucleoplasm; cytoplasmic vesicle membrane; nucleolus; plasma membrane

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; forebrain neuron differentiation; FasL biosynthetic process