Toxoplasma Gondii Rhoptry protein Recombinant Protein | TOXGO ROP4 recombinant protein
Toxoplasma Gondii Rhoptry protein Recombinant
Purity
>95%, as determined by SDS-PAGE.
Synonyms
Toxoplasma Gondii Rhoptry protein; Toxoplasma Gondii Rhoptry protein Recombinant; toxoplasma gondii; rop4; Rhoptry; toxgo; TOXGO ROP4 recombinant protein
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE.
Form/Format
Lyophilized from 50mM Tris-HCl, 60mM NaCl and 50% glycerol.
Sequence Length
578
Application Notes
Toxoplasma ROP4 antigen is suitable for ELISA and Western blots, excellent antigen for detection of Toxoplasma with minimal specificity problems.
Host Note
DNA sequence encoding immunodominant fragment of Toxoplasma ROP4 protein was expressed in Escherichia Coli.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant TOXGO ROP4 encodes the immunodominant sequences of the Toxoplasma Dense Granule protein.
Recombinant ROP4 contains an N terminal GST purification tag and migrates as an approximately 43 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Recombinant ROP4 contains an N terminal GST purification tag and migrates as an approximately 43 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for TOXGO ROP4 recombinant protein
ROP4, a previously uncharacterized member of the ROP2 family of proteins. ROP4 is secreted from the rhoptries during or shortly after invasion and associates with the PVM, presumably through its predicted transmembrane domain. ROP4 becomes phosphorylated in the infected cell, by either host cell protein kinase (s) or parasite kinase (s) activated by host cell factors.
Product Categories/Family for TOXGO ROP4 recombinant protein
NCBI and Uniprot Product Information
Uniprot Description
Function: Thought to play a role in parasitophorous vacuole membrane function during the infection of host organisms. Ref.1
Subcellular location: Secreted. Parasitophorous vacuole membrane. Note: Localized to rhoptries which are secretory organelles at the apical end of the parasite and secreted during host cell invasion when it becomes associated with the parasitophorous vacuole membrane. Ref.1
Post-translational modification: Phosphorylated on multiple serine and threonine residues in parasitic extracts and infected cells but not in extracellular parasites. Ref.1
Sequence similarities: Contains 1 protein kinase domain.
Caution: Lacks the active site residue so is predicted to be catalytically inactive. Ref.1
Similar Products
Product Notes
The TOXGO ROP4 rop4 (Catalog #AAA553140) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Toxoplasma ROP4 antigen is suitable for ELISA and Western blots, excellent antigen for detection of Toxoplasma with minimal specificity problems. . Researchers should empirically determine the suitability of the TOXGO ROP4 rop4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Toxoplasma Gondii Rhoptry protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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