Rho Associated Coiled Coil Containing Protein Kinase 2 Recombinant Protein | Rock2 recombinant protein

Recombinant Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2)

Cat. Num. AAA2029508

• Gene Names: ROCK2; ROCK-II
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Rho Associated Coiled Coil Containing Protein Kinase 2; Recombinant Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2); Rock2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.7 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LQDERDS LAAQLEITLT KADSEQLARS IAEEQYSDLE KEKIMKELEI KEMMARHKQE LTEKDATIAS LEETNRTLTS DVANLANEKE ELNNKLKDVQ EQLSRLKDEE ISAAAIKAQF EKQLLTERTL KTQAVNKLAE IMNRKEPVKR GNDTDVRRKE KENRKLHMEL KSEREKLTQQ MIKYQKELNE MQAQIAEESQIRIELQMTLD SKDSDIEQLR SQLQALHIGL DSSSIGSGPG
• Sequence Length: 1384

• Applicable Applications for Rock2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.1kDa
• Accurate Molecular Mass (KD): 31kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Leu904~Gly1140
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119621342
• NCBI GeneID: 9475
• Molecular Weight: 160,900 Da
• NCBI Official Full Name: Rho-associated, coiled-coil containing protein kinase 2, isoform CRA_a
• NCBI Official Synonym Full Names: Rho-associated, coiled-coil containing protein kinase 2
• NCBI Official Symbol: ROCK2
• NCBI Official Synonym Symbols: ROCK-II
• NCBI Protein Information: rho-associated protein kinase 2
• UniProt Protein Name: Rho-associated protein kinase 2
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: ROCK2
• UniProt Synonym Gene Names: KIAA0619; ROCK-II
• UniProt Entry Name: ROCK2_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Uniprot Description

ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: centrosome; plasma membrane; spindle pole centrosome; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; structural molecule activity; ATP binding

Biological Process: regulation of cell adhesion; axon guidance; dendrite morphogenesis; rhythmic process; cytokinesis; regulation of circadian rhythm; protein amino acid phosphorylation; Rho protein signal transduction; negative regulation of angiogenesis; smooth muscle contraction; induction of apoptosis via death domain receptors; regulation of actin cytoskeleton organization and biogenesis; regulation of stress fiber formation; regulation of focal adhesion formation; neural tube closure; ephrin receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; regulation of keratinocyte differentiation; centrosome duplication