Mouse Rho-associated protein kinase 2 ELISA Kit | Rock2 elisa kit

Mouse Rho-associated protein kinase 2 ELISA Kit

Cat. Num. AAA2887192

• Gene Names: Rock2; Rock2m; Rock-II; ROKalpha; mKIAA0619; Rho-kinase; B230113H15Rik
• Reactivity: Mouse
• Synonyms: Rho-associated protein kinase 2; Mouse Rho-associated protein kinase 2 ELISA Kit; Rho-associated; coiled-coil-containing protein kinase 2; coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2; Rock2; 2.7.11.1; Rock2 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Rho-associated protein kinase 2. No significant cross-reactivity or interference was observed.
• Sequence Length: 1388

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 1.56 ng/mL -100 ng/mL
• Sensitivity: <0.78 ng/mL.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Rock2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse Rho-associated protein kinase 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho-associated protein kinase 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rho-associated protein kinase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho-associated protein kinase 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rho-associated protein kinase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 134949013
• NCBI GeneID: 19878
• NCBI Accession #: NP_033098.2
• NCBI GenBank Nucleotide #: NM_009072.2
• UniProt Accession #: P70336; Q8BR64; Q8CBR0; A5XDA7
• Molecular Weight: 166,760 Da
• NCBI Official Full Name: rho-associated protein kinase 2
• NCBI Official Synonym Full Names: Rho-associated coiled-coil containing protein kinase 2
• NCBI Official Symbol: Rock2
• NCBI Official Synonym Symbols: Rock2m; Rock-II; ROKalpha; mKIAA0619; Rho-kinase; B230113H15Rik
• NCBI Protein Information: rho-associated protein kinase 2
• UniProt Protein Name: Rho-associated protein kinase 2
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: Rock2
• UniProt Synonym Gene Names: ROCK-II
• UniProt Entry Name: ROCK2_MOUSE

Uniprot Description

ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Kinase, protein; Protein kinase, AGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; DMPK family; ROCK subfamily

Cellular Component: centrosome; cytoplasm; cytoskeleton; membrane; nucleus; plasma membrane; spindle pole centrosome

Molecular Function: ATP binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; Rho GTPase binding; transferase activity

Biological Process: actin cytoskeleton organization and biogenesis; centrosome duplication; cortical actin cytoskeleton organization and biogenesis; dendrite morphogenesis; I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; negative regulation of angiogenesis; neural tube closure; phosphorylation; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of circadian rhythm; regulation of keratinocyte differentiation; Rho protein signal transduction; rhythmic process; smooth muscle contraction