Human Polycomb Group Ring Finger Protein 4 (PCGF4) ELISA Kit | PCGF4 elisa kit

Human Polycomb Group Ring Finger Protein 4 (PCGF4) ELISA Kit

Cat. Num. AAA452719

• Reactivity: Human
• Synonyms: Polycomb Group Ring Finger Protein 4 (PCGF4); Human Polycomb Group Ring Finger Protein 4 (PCGF4) ELISA Kit; CAAT box DNA-binding protein subunit C; NF-YC; Nuclear transcription factor Y subunit C; Nuclear transcription factor Y subunit gamma; Transactivator HSM-1/2; PCGF4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PCGF4. No significant cross-reactivity or interference between PCGF4 and analogues was observed.
• Sequence Length: 469

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.122ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PCGF4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PCGF4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCGF4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PCGF4 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCGF4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PCGF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCGF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PCGF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 323276616
• NCBI GeneID: 100532731
• NCBI Accession #: NP_001190991.1
• NCBI GenBank Nucleotide #: NM_001204062.1
• UniProt Accession #: P35226; Q16030; Q5T8Z3; Q96F37
• Molecular Weight: 36,949 Da
• NCBI Official Full Name: COMMD3-BMI1 read-through protein
• NCBI Official Synonym Full Names: COMMD3-BMI1 readthrough
• NCBI Official Symbol: COMMD3-BMI1
• NCBI Protein Information: COMMD3-BMI1 read-through protein
• UniProt Protein Name: Polycomb complex protein BMI-1
• UniProt Gene Name: BMI1
• UniProt Synonym Gene Names: PCGF4; RNF51

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]

Uniprot Description

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.