Rabbit anti-Human, Mouse NKX2-1 Polyclonal Antibody | anti-NKX2-1 antibody

NKX2-1, NT (NKX2-1, NKX2A, TITF1, TTF1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1) (Azide free) (HRP)

Cat. Num. AAA6325547

• Gene Names: NKX2-1; BCH; BHC; NK-2; TEBP; TTF1; NKX2A; TITF1; TTF-1; NKX2.1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: NKX2-1; Polyclonal Antibody; NT (NKX2-1; NKX2A; TITF1; TTF1; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1) (Azide free) (HRP); Thyroid transcription factor 1; anti-NKX2-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NKX2-1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NKX2-1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human NKX2-1, UniProt Accession #P43699. Species sequence homology: rat
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS6006895. This demonstrates that MBS6006895 detected the NKX2-1 protein (arrow).)

Testing Data

(All lanes: MBS6006895 (1:2000) Lane 1: mouse lung lysate Lane 2: TT whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Predicted band size: 39kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: MBS6006895 (1:2000); Lane 1: Hela whole cell lysate; Lane 2: human fetal lung lysate; Lane 3: human lung lysate; Lane 4: TT whole cell lysate; Lane 5: rat thyroid gland lysate; Lane 6: mouse lung lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated. Predicted band size: 39kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NKX2-1 antibody

This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-NKX2-1 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Kim, J.H., et al. Acta Cytol. 54(3):277-282(2010). Xu, B., et al. Appl. Immunohistochem. Mol. Morphol. 18(3):244-249(2010). Narumi, S., et al. J. Clin. Endocrinol. Metab. 95(4):1981-1985(2010). Guillot, L., et al. Hum. Mutat. 31 (2), E1146-E1162 (2010): Cantara, S., et al. Thyroid Res 3 (1), 4 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4507715
• NCBI GeneID: 7080
• NCBI Accession #: NP_003308.1
• NCBI GenBank Nucleotide #: NM_003317.3
• UniProt Accession #: P43699; O14954; O14955; Q7KZF6; Q9BRJ8; D3DSA3; O14956; Q13306
• Molecular Weight: 38,596 Da
• NCBI Official Full Name: homeobox protein Nkx-2.1 isoform 2
• NCBI Official Synonym Full Names: NK2 homeobox 1
• NCBI Official Symbol: NKX2-1
• NCBI Official Synonym Symbols: BCH; BHC; NK-2; TEBP; TTF1; NKX2A; TITF1; TTF-1; NKX2.1
• NCBI Protein Information: homeobox protein Nkx-2.1; NK-2 homolog A; OTTHUMP00000178847; OTTHUMP00000225297; thyroid nuclear factor 1; thyroid transcription factor 1; homeobox protein NK-2 homolog A
• UniProt Protein Name: Homeobox protein Nkx-2.1
• Protein Family: Homeobox protein
• UniProt Gene Name: NKX2-1
• UniProt Synonym Gene Names: NKX2A; TITF1; TTF1; TTF-1; T/EBP
• UniProt Entry Name: NKX21_HUMAN

Uniprot Description

NKX2-1: Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC); also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). This syndrome include neurological, thyroid, and respiratory problems. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; axon guidance; positive regulation of transcription, DNA-dependent; rhythmic process; neuron migration; response to hormone stimulus; globus pallidus development; locomotory behavior; forebrain dorsal/ventral pattern formation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of circadian rhythm; Leydig cell differentiation; phospholipid metabolic process; forebrain development; thyroid gland development; oligodendrocyte differentiation; anatomical structure formation; forebrain neuron fate commitment; negative regulation of cell migration; hippocampus development; induction; cerebral cortex GABAergic interneuron differentiation; cerebral cortex cell migration; pituitary gland development; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; endoderm development; lung development

Disease: Thyroid Carcinoma, Papillary; Chorea, Benign Hereditary; Choreoathetosis And Congenital Hypothyroidism With Or Without Pulmonary Dysfunction