Human roundabout, axon guidance receptor, homolog 1 (Drosophila) ELISA Kit | ROBO1 elisa kit

Human Roundabout homolog 1, ROBO1 ELISA Kit

Cat. Num. AAA921385

• Gene Names: ROBO1; SAX3; DUTT1
• Reactivity: Human
• Synonyms: roundabout; axon guidance receptor; homolog 1 (Drosophila); Human Roundabout homolog 1; ROBO1 ELISA Kit; Human Roundabout homolog 1 (ROBO1) ELISA kit; DUTT1; FLJ21882; MGC131599; MGC133277; SAX3; roundabout 1; ROBO1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ROBO1. No significant cross-reactivity or interference between human ROBO1 and analogues was observed.
• Sequence Length: 1551

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ROBO1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ROBO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ROBO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROBO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROBO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 224994173
• NCBI GeneID: 6091
• NCBI Accession #: NP_001139317.1
• NCBI GenBank Nucleotide #: NM_001145845.1
• UniProt Accession #: Q9Y6N7; Q1RMC7; Q7Z300; Q9BUS7; B2RXI1; D3DU36; E9PD49
• Molecular Weight: 180,930 Da
• NCBI Official Full Name: roundabout homolog 1 isoform d
• NCBI Official Synonym Full Names: roundabout, axon guidance receptor, homolog 1 (Drosophila)
• NCBI Official Symbol: ROBO1
• NCBI Official Synonym Symbols: SAX3; DUTT1
• NCBI Protein Information: roundabout homolog 1; deleted in U twenty twenty
• UniProt Protein Name: Roundabout homolog 1
• Protein Family: Roundabout
• UniProt Gene Name: ROBO1
• UniProt Synonym Gene Names: DUTT1
• UniProt Entry Name: ROBO1_HUMAN

NCBI Description

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

ROBO1: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. Belongs to the immunoglobulin superfamily. ROBO family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p12

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; axolemma

Molecular Function: identical protein binding; protein binding; axon guidance receptor activity; LRR domain binding

Biological Process: caspase activation; nervous system development; axon guidance; negative regulation of negative chemotaxis; cell migration during sprouting angiogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; positive regulation of axonogenesis; cell adhesion; homophilic cell adhesion; axon midline choice point recognition; negative regulation of mammary gland epithelial cell proliferation