Ring Finger Protein 4 (RNF4) Recombinant Protein | RNF4 recombinant protein

Recombinant Ring Finger Protein 4 (RNF4)

Cat. Num. AAA2086797

• Gene Names: Rnf4; SNURF; Gtrgeo8; AU018689
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Ring Finger Protein 4 (RNF4); Recombinant Ring Finger Protein 4 (RNF4); RES4-26; SNURF; E3 ubiquitin-protein ligase RNF4; Small nuclear ring finger protein; RNF4 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 194

• Applicable Applications for RNF4 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Met1~Ile194
• Tag: Two N-terminal Tags, His-tag and GST-tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Prokaryotic expression
• Organism Species: Mouse
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 7.6
• Subcellular Location: Cytoplasm. Nucleus. Pml Body. Nucleoplasm.
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Product Categories/Family for RNF4 recombinant protein

Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 747165403
• NCBI GeneID: 19822
• NCBI Accession #: NP_001291198.1
• NCBI GenBank Nucleotide #: NM_001304269.1
• UniProt Accession #: Q9QZS2; O35941; Q541Z6
• Molecular Weight: 51.9kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF4
• NCBI Official Synonym Full Names: ring finger protein 4
• NCBI Official Symbol: Rnf4
• NCBI Official Synonym Symbols: SNURF; Gtrgeo8; AU018689
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF4
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF4
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Rnf4

NCBI Description

This gene encodes a small nuclear RING finger protein that mediates ubiquitylation of polysumoylated proteins. Deficiency of the encoded protein in mice leads to embryonic lethality and global DNA hypermethylation. A similar protein in humans is required for arsenic-induced degradation of promyelocytic leukemia protein in acute promyelocytic leukemia. Alternative splicing of this gene results in multiple transcript variants. A pseudogene for this gene has been identified on chromosome 10. [provided by RefSeq, Jan 2015]

Uniprot Description

RNF4: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation.

Protein type: EC 6.3.2.-; Ligase; Nuclear receptor co-regulator; Transcription factor; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 5|5 B2

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; PML body

Molecular Function: androgen receptor binding; DNA binding; estrogen receptor binding; nucleosome binding; progesterone receptor binding; protein binding; protein homodimerization activity; SUMO polymer binding; TATA-binding protein binding; transcription factor binding; ubiquitin conjugating enzyme binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: negative regulation of protein sumoylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein ubiquitination; response to arsenic