E3 ubiquitin-protein ligase RNF4 (Rnf4) Recombinant Protein | Rnf4 recombinant protein

Recombinant Mouse E3 ubiquitin-protein ligase RNF4 (Rnf4)

Cat. Num. AAA1392596

• Gene Names: Rnf4; SNURF; Gtrgeo8; AU018689
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase RNF4 (Rnf4); Recombinant Mouse E3 ubiquitin-protein ligase RNF4 (Rnf4); Rnf4 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-194, full length protein
• Sequence: MSTRNPQRKRRGGTVNSRQTQKRTRETTSTPEVSLETEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSRDKDVYVTTHTPRSTKDDGATGPRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI
• Sequence Length: 194

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Rnf4 recombinant protein

This protein contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278
PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 747165403
• NCBI GeneID: 19822
• NCBI Accession #: NP_001291198.1
• NCBI GenBank Nucleotide #: NM_001304269.1
• UniProt Accession #: Q9QZS2; O35941; Q541Z6
• Molecular Weight: 21,911 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF4
• NCBI Official Synonym Full Names: ring finger protein 4
• NCBI Official Symbol: Rnf4
• NCBI Official Synonym Symbols: SNURF; Gtrgeo8; AU018689
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF4
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF4
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Rnf4

NCBI Description

This gene encodes a small nuclear RING finger protein that mediates ubiquitylation of polysumoylated proteins. Deficiency of the encoded protein in mice leads to embryonic lethality and global DNA hypermethylation. A similar protein in humans is required for arsenic-induced degradation of promyelocytic leukemia protein in acute promyelocytic leukemia. Alternative splicing of this gene results in multiple transcript variants. A pseudogene for this gene has been identified on chromosome 10. [provided by RefSeq, Jan 2015]

Uniprot Description

E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.