Ribonuclease L (RNASEL) Recombinant Protein | RNASEL recombinant protein

Recombinant Ribonuclease L (RNASEL)

Cat. Num. AAA2011540

• Gene Names: RNASEL; RNS4; PRCA1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ribonuclease L (RNASEL); Recombinant Ribonuclease L (RNASEL); RNASEL recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-IGK LKFFIDEKYK IADTSEGGIY LGFYEKQEVA VKTFCEGSPR AQREVSCLQS SRENSHLVTF YGSESHRGHL FVCVTLCEQT LEACLDVHRG EDVENEEDEF ARNVLSSIFK AVQELHLSCG YTHQDLQPQN ILIDSKKAAH LADFDKSIKW AGDPQEVKRD LEDLGRLVLY VVKKGSISFE DLKAQSNEEV VQLSPDEETK DLIHRLFHPG EHVRDCLSDL LGH
• Sequence Length: 741

• Applicable Applications for RNASEL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ile358~His583 (Accession # Q05823) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 10863929
• NCBI GeneID: 6041
• NCBI Accession #: NP_066956.1
• NCBI GenBank Nucleotide #: NM_021133.3
• UniProt Accession #: Q05823; Q5W0L2
• Molecular Weight: 27.2kDa
• NCBI Official Full Name: 2-5A-dependent ribonuclease
• NCBI Official Synonym Full Names: ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
• NCBI Official Symbol: RNASEL
• NCBI Official Synonym Symbols: RNS4; PRCA1
• NCBI Protein Information: 2-5A-dependent ribonuclease; RNase L; ribonuclease 4; 2-5A-dependent RNase; interferon-induced 2-5A-dependent RNase; 2',5'-oligoisoadenylate synthetase-dependent
• UniProt Protein Name: 2-5A-dependent ribonuclease
• Protein Family: 2-5A-dependent ribonuclease
• UniProt Gene Name: RNASEL
• UniProt Synonym Gene Names: RNS4; 2-5A-dependent RNase; RNase L
• UniProt Entry Name: RN5A_HUMAN

NCBI Description

This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]

Uniprot Description

RNASEL: a multifunctional enzyme that possesses protein kinase and endoribonuclease activity, Mediator of interferon action, which play a role in mediating resistance to virus infection and apoptosis. Might play a central role in the regulation of mRNA turnover.

Protein type: Ribonuclease; Protein kinase, Other; EC 3.1.26.-; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; Other-Unique family

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: nuclear matrix; mitochondrion; cytosol

Molecular Function: endoribonuclease activity; rRNA binding; protein binding; RNA binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: negative regulation of viral genome replication; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; mRNA processing; defense response to virus; protein amino acid phosphorylation; rRNA processing

Disease: Prostate Cancer, Hereditary, 1