Rat ribonuclease, RNase A family, 3 (eosinophil cationic protein) ELISA Kit | RNASE3 elisa kit

Rat eosinophil Cationic protein, ECP ELISA Kit

Cat. Num. AAA703318

• Gene Names: Ear11; R1; R7; EAR; ECP; Rnase3
• Reactivity: Rat
• Synonyms: ribonuclease; RNase A family; 3 (eosinophil cationic protein); Rat eosinophil Cationic protein; ECP ELISA Kit; Rat eosinophil cationic protein; ECP; RNS3; cytotoxic ribonuclease; eosinophil cationic protein; RNASE3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat RNASE3. No significant cross-reactivity or interference between Rat RNASE3 and analogues was observed.
• Sequence Length: 155

• Samples: Serum, plasma and tissue homogenates
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RNASE3 elisa kit

Introduction: Eosinophil cationic protein (ECP) is a small, basic protein found in the matrix of the eosinophil's large specific granule that has cytotoxic, helminthotoxic, and ribonuclease activity, and is a member of the ribonuclease multigene family. Human eosinophil granules contain several basic proteins including ECP, eosinophil-derived neurotoxin (EDN) and major basic protein (MBP). ECP and MBP are potent helminthotoxins while EDN is less so. Both ECP and EDN possess neurotoxic and ribonuclease activities

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ECP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ECP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ECP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ECP in the samples is then determined by comparing the O.D. of the samples to the standard curve

NCBI and Uniprot Product Information

• NCBI GI #: 20302099
• NCBI GeneID: 192264
• NCBI Accession #: NP_620257.1
• NCBI GenBank Nucleotide #: NM_138902.1
• UniProt Accession #: P70709
• Molecular Weight: 18,007 Da
• NCBI Official Full Name: eosinophil cationic protein
• NCBI Official Synonym Full Names: eosinophil-associated, ribonuclease A family, member 11
• NCBI Official Symbol: Ear11
• NCBI Official Synonym Symbols: R1; R7; EAR; ECP; Rnase3
• NCBI Protein Information: eosinophil cationic protein; EAR-11; RNase 3; ribonuclease 1; ribonuclease 3; ribonuclease 7; eosinophil-associated ribonuclease; eosinophil secondary granule ribonuclease 11
• UniProt Protein Name: Eosinophil cationic protein
• Protein Family: Eosinophil cationic protein
• UniProt Gene Name: Rnase3
• UniProt Synonym Gene Names: Ear11; Rns3; ECP; EAR-11; RNase 3
• UniProt Entry Name: ECP_RAT

NCBI Description

cationic protein with similarity to human ECP; also has similarity to human eosinophil-derived neurotoxin and human ribonuclease k6 [RGD, Feb 2006]

Uniprot Description

Function: Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities