Guinea Pig Eosinophil cationic protein ELISA Kit | ECP elisa kit

Guinea Pig Eosinophil cationic protein ELISA Kit

Cat. Num. AAA732095

• Gene Names: RNASE3; ECP; RNS3
• Reactivity: Guinea Pig
• Synonyms: Eosinophil cationic protein; Guinea Pig Eosinophil cationic protein ELISA Kit; ECP elisa kit

• Reactivity: Guinea Pig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This ECP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea Pig ECP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ECP elisa kit

Principle of the Assay: ECP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ECP. Standards or samples are then added to the microtiter plate wells and ECP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ECP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ECP are added to each well to "sandwich" the ECP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ECP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45243507
• NCBI GeneID: 6037
• NCBI Accession #: NP_002926.2
• NCBI GenBank Nucleotide #: NM_002935.2
• UniProt Accession #: P12724; Q4VBC1; Q8WTP7; Q8WZ62; Q9GZN9
• Molecular Weight: 18,385 Da
• NCBI Official Full Name: eosinophil cationic protein
• NCBI Official Synonym Full Names: ribonuclease, RNase A family, 3
• NCBI Official Symbol: RNASE3
• NCBI Official Synonym Symbols: ECP; RNS3
• NCBI Protein Information: eosinophil cationic protein; RNase 3; ribonuclease 3; cytotoxic ribonuclease
• UniProt Protein Name: Eosinophil cationic protein
• Protein Family: Eosinophil cationic protein
• UniProt Gene Name: RNASE3
• UniProt Synonym Gene Names: ECP; RNS3; ECP; RNase 3
• UniProt Entry Name: ECP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; Secreted; Ribonuclease; EC 3.1.27.-

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space; extracellular region

Molecular Function: nucleic acid binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response