Receptor-interacting serine/threonine-protein kinase 3 (Ripk3) Recombinant Protein | Ripk3 recombinant protein

Recombinant rat Receptor-interacting serine/threonine-protein kinase 3 (Ripk3)

Cat. Num. AAA7135872

• Gene Names: Ripk3; Rip3; Hcyp2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Receptor-interacting serine/threonine-protein kinase 3 (Ripk3); Recombinant rat Receptor-interacting serine/threonine-protein kinase 3 (Ripk3); Homocysteine respondent protein HCYP2; (RIP-like protein kinase 3); (Receptor-interacting protein 3); (RIP-3); (Rip3); Ripk3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid or Lyophilized powder; If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.; If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, p
• Sequence Positions: 1-478aa; Full Length
• Sequence: MSSVKLWLNGASSISLVGSEELENLGFVGKGGFGAVFRARHTAWNLDVAVKIVNSKKISREVKAMVNLRHENVLLLLGVTENLEWDYVYGPALVTGFMENGSLSGLLQPSCPRPWPLLCRLLEEVVLGMCYLHSLNPSLLHRDLKPSNVLLDPELHAKLADFGLSTFQGGSQSGSGSGSRDSGGTLAYLAPELLDNDGKASKASDVYSFGVLVWTVLAGREAEVVDKTSLIRGAVCNRQRRPPLTELPPDSPETPGLEGLKELMTHCWSSEPKDRPSFQDCESKTNNVYILVQDKVDAAVSKVKHYLSQYRSSDTKLSARESSQKGTEVDCPRETIVYEMLDRLHLEEPSGSVPERLTSLTERRGKEASFGHATPAGTSSDTLAGTPQIPHTLPSRGTTPRPAFTETPGPDPQRNQGDGRNSNPWYTWNAPNPMTGLQSIVLNNCSEVQIGQHNCMSVQPRTAFPKKEPAQFGRGRGW
• Sequence Length: 478

• Species: Rat
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. ; Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C.; The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.; Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for Ripk3 recombinant protein

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

Product Categories/Family for Ripk3 recombinant protein

Cancer

References

"A homocysteine-respondent gene cloned from WKY VSMCs by differential display."
Chen K.H., Tang J. Submitted (MAY-2001)

NCBI and Uniprot Product Information

• NCBI GI #: 21326477
• NCBI GeneID: 246240
• NCBI Accession #: NP_647558.1
• NCBI GenBank Nucleotide #: NP_647558.1
• UniProt Accession #: Q9Z2P5
• Molecular Weight: 56.1
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 3
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 3
• NCBI Official Symbol: Ripk3
• NCBI Official Synonym Symbols: Rip3; Hcyp2
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: Ripk3
• UniProt Synonym Gene Names: Rip3; RIP-3
• UniProt Entry Name: RIPK3_RAT

NCBI Description

a putative homocysteine respondent protein [RGD, Feb 2006]

Uniprot Description

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; RIPK family

Cellular Component: cytosol; intracellular; mitochondrion; plasma membrane

Molecular Function: ATP binding; identical protein binding; NF-kappaB-inducing kinase activity; protein complex binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of NF-kappaB transcription factor; activation of protein kinase activity; I-kappaB kinase/NF-kappaB cascade; lymph node development; positive regulation of ligase activity; positive regulation of oxidoreductase activity; protein amino acid autophosphorylation; protein heterooligomerization; protein homooligomerization; regulation of activated T cell proliferation; regulation of adaptive immune response; regulation of interferon-gamma production; regulation of T cell mediated cytotoxicity; spleen development; T cell differentiation in the thymus; T cell homeostasis; thymus development