Transcription termination factor Rho Recombinant Protein | rho recombinant protein

Recombinant E Coli Transcription termination factor Rho

Cat. Num. AAA1265378

• Gene Names: rho; ECK3775; hdf; JW3756; nitA; nusD; psuA; rnsC; sbaA; sun; tabC; tsu
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Transcription termination factor Rho; Recombinant E Coli Transcription termination factor Rho; ATP-dependent helicase Rho; rho recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 1-419aa; Full Length
• Sequence: MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTARVLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNTVVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLINKLAMTKTNDDFFEMMKRS
• Sequence Length: 419

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for rho recombinant protein

Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPase which utilizes all four ribonucleoside triphosphates as substrates.

References

The nucleotide sequence of the rho gene of E. coli K-12.Pinkham J.L., Platt T.Nucleic Acids Res. 11:3531-3545(1983) Analysis of the Escherichia coli genome DNA sequence of the region from 84.5 to 86.5 minutes.Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.Science 257:771-778(1992) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Autogenous regulation of the gene for transcription termination factor rho in Escherichia coli localization and function of its attenuators.Matsumoto Y., Shigesada K., Hirano M., Imai M.J. Bacteriol. 166:945-958(1986) Cloning and expression of the rfe-rff gene cluster of Escherichia coli.Ohta M., Ina K., Kusuzaki K., Kido N., Arakawa Y., Kato N.Mol. Microbiol. 5:1853-1862(1991) The ts15 mutation of Escherichia coli alters the sequence of the C-terminal nine residues of Rho protein.Opperman T., Martinez A., Richardson J.P.Gene 152:133-134(1995) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.Link A.J., Robison K., Church G.M.Electrophoresis 18:1259-1313(1997) Mutations in an RNP1 consensus sequence of Rho protein reduce RNA binding affinity but facilitate helicase turnover.Brennan C.A., Platt T.J. Biol. Chem. 266:17296-17305(1991) Site-directed alterations in the ATP-binding domain of rho protein affect its activities as a termination factor.Dombroski A.J., Brennan C.A., Spear P., Platt T.J. Biol. Chem. 263:18802-18809(1988) Bicyclomycin and dihydrobicyclomycin inhibition kinetics of Escherichia coli rho-dependent transcription termination factor ATPase activity.Park H.G., Zhang X., Moon H.S., Zwiefka A., Cox K., Gaskell S.J., Widger W.R., Kohn H.Arch. Biochem. Biophys. 323:447-454(1995) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) 1H, 15N and 13C resonance assignments and secondary structure determination of the RNA-binding domain of E Coli rho protein.Briercheck D.M., Allison T.J., Richardson J.P., Ellena J.F., Wood T.C., Rule S.G.J. Biomol. NMR 8:429-444(1996) The NMR structure of the RNA binding domain of E. coli rho factor suggests possible RNA-protein interactions.Briercheck D.M., Allison T.J., Wood T.C., Richardson J.P., Rule G.S.Nat. Struct. Biol. 5:393-399(1998) Crystal structure of the RNA-binding domain from transcription termination factor rho.Allison T.J., Wood T.C., Briercheck D.M., Rastinejad F., Richardson J.P., Rule G.S.Nat. Struct. Biol. 5:352-356(1998) The structural basis for terminator recognition by the Rho transcription termination factor.Bogden C.E., Fass D., Bergman N., Nichols M.D., Berger J.M.Mol. Cell 3:487-493(1999) Structure of the Rho transcription terminator mechanism of mRNA recognition and helicase loading.Skordalakes E., Berger J.M.Cell 114:135-146(2003) Structural mechanism of inhibition of the Rho transcription termination factor by the antibiotic bicyclomycin.Skordalakes E., Brogan A.P., Park B.S., Kohn H., Berger J.M.Structure 13:99-109(2005) Running in reverse the structural basis for translocation polarity in hexameric helicases.Thomsen N.D., Berger J.M.Cell 139:523-534(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 16131639
• NCBI GeneID: 948297
• NCBI Accession #: NP_418230.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AG30; P03002; Q2M891; Q48357
• Molecular Weight: 51.0 kDa
• NCBI Official Full Name: transcription termination factor
• NCBI Official Symbol: rho
• NCBI Official Synonym Symbols: ECK3775; hdf; JW3756; nitA; nusD; psuA; rnsC; sbaA; sun; tabC; tsu
• NCBI Protein Information: transcription termination factor
• UniProt Protein Name: Transcription termination factor Rho
• Protein Family: Transcription termination factor
• UniProt Gene Name: rho
• UniProt Synonym Gene Names: nitA; psuA; rnsC; sbaA; tsu
• UniProt Entry Name: RHO_ECOLI

NCBI Description

rho has been shown to be an essential gene (Das, 1976; Bubunenko, 2007). [More information is available at EcoGene: EG10845]. Rho is required for one of the two major types of termination of RNA transcription. [More information is available at EcoCyc: EG10845].

Uniprot Description

Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPase which utilizes all four ribonucleoside triphosphates as substrates.