Raft Linking Protein (Raftlin) Recombinant Protein | Raftlin recombinant protein

Recombinant Raft Linking Protein (Raftlin)

Cat. Num. AAA2018762

• Gene Names: Rftn1; AI853526; 2310015N21Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Raft Linking Protein (Raftlin); Recombinant Raft Linking Protein (Raftlin); Raftlin recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AAET DAATAGVNRR PETKPGSTGD VPSAQQPGIP SPSAENEAGE FPLRGLQPAL DRSEGDPSNG PEELPSRKME IFAFFNRPKS QQKCRQYYPV TIPLQVSKNG QTVSSLDASW LEHMSDHFRK GGVLVNAVFQ LGMANDSFYG LTDGVFIFEA VSTEDNRTTQ GYDAIVVEQW TVLEGTEVQT DYMPLLNSLAAYGWQLTCVL PTPILKTTRE GNVSTKQIVF LQRPCLPQKT KKRESKFQWR F
• Sequence Length: 554

• Applicable Applications for Raftlin recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ala207~Phe451 (Accession # Q6A0D4) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Lipid-anchor
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.5
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 33859813
• NCBI GeneID: 76438
• NCBI Accession #: NP_852062.1
• NCBI GenBank Nucleotide #: NM_181397.2
• UniProt Accession #: Q6A0D4; Q3U654; Q3U8P6; Q80US1
• Molecular Weight: 31.0kDa
• NCBI Official Full Name: raftlin
• NCBI Official Synonym Full Names: raftlin lipid raft linker 1
• NCBI Official Symbol: Rftn1
• NCBI Official Synonym Symbols: AI853526; 2310015N21Rik
• NCBI Protein Information: raftlin; raft-linking protein
• UniProt Protein Name: Raftlin
• UniProt Gene Name: Rftn1
• UniProt Synonym Gene Names: Kiaa0084
• UniProt Entry Name: RFTN1_MOUSE

Uniprot Description

RFTN1: May play a pivotal role in the formation and/or maintenance of lipid rafts. May regulate B-cell antigen receptor- mediated signaling. Belongs to the raftlin family.

Protein type: Unknown function

Cellular Component: protein complex; membrane; cell; cytoplasm; plasma membrane; intracellular; endosome; lipid raft

Molecular Function: double-stranded RNA binding

Biological Process: protein transport into lipid raft; B cell receptor signaling pathway; response to exogenous dsRNA; T cell antigen processing and presentation; interleukin-17 production; dsRNA transport; toll-like receptor 3 signaling pathway; lipid raft formation; T cell receptor signaling pathway; growth