Immunohistochemistry (IHC)
(MBS9215640 staining FEV in human smallintestine sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated withprimary antibody (1/25) for 1 hours at 37°C. Aundiluted biotinylated goat polyvalent antibodywas used as the secondary antibody.)
Rabbit anti-Human FEV Polyclonal Antibody | anti-FEV antibody
FEV Antibody (N-Term)
WB~~1:2000
Immunohistochemistry (IHC)
(MBS9215640 staining FEV in human smallintestine sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated withprimary antibody (1/25) for 1 hours at 37°C. Aundiluted biotinylated goat polyvalent antibodywas used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9215640 staining FEV in human smallintestine sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated withprimary antibody (1/25) for 1 hours at 37°C. Aundiluted biotinylated goat polyvalent antibodywas used as the secondary antibody.)
Testing Data (TD)
(Anti-FEV Antibody (N-Term) at 1:2000 dilution+ K562 whole cell lysate Lysates/proteins at 20?g per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(Anti-FEV Antibody (N-Term) at 1:2000 dilution+ K562 whole cell lysate Lysates/proteins at 20?g per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations. [provided by RefSeq, Jul 2008]
Uniprot Description
FEV: Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth. Genetic variation in FEV may be associated with susceptibility to sudden infant death syndrome (SIDS). SIDS remains elusive in its causes and devastating in its consequences. Despite the impressive decline in the incidence of SIDS since the recommendation to avoid the prone sleep position, SIDS remains a leading cause of death in the first year of life. A chromosomal aberration involving FEV is found in Ewing tumors. Translocation t(2;21;22)(q23;q22;q12) that forms a EWSR1-FEV fusion protein with a potential oncogenic activity. Belongs to the ETS family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 2q36
Cellular Component: nucleus
Molecular Function: sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity; transcription corepressor activity
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; neuron fate specification; cell differentiation; neuron maturation
Research Articles on FEV
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Product Notes
The FEV fev (Catalog #AAA9215640) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FEV Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FEV can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry -Paraffin, (IHC-P), Western Blot(WB), ELISA (EIA). IHC-P~~1:25 WB~~1:2000. Researchers should empirically determine the suitability of the FEV fev for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FEV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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