Raft Linking Protein Recombinant Protein | Raftlin recombinant protein

Recombinant Raft Linking Protein (Raftlin)

Cat. Num. AAA2030554

• Gene Names: RFTN1; MIG2; PIG9; PIB10; RAFTLIN
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Raft Linking Protein; Recombinant Raft Linking Protein (Raftlin); Raftlin recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.7 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SHQ KCRQYYPVTI PLHVSKNGQT VSGLDANWLE HMSDHFRKGG MLVNAVFYLG IVNDSLHGLT DGVFIFEAVS TEDSKTIQGY DAIVVEQWTV LEGVEVQTDY VPLLNSLAAY GWQLTCVLPT PVVKTTSEGS VSTKQIVFLQ RPCLPQKIKK KESKFQWRFS REEMHNRQMR KSKGKLSARD KQQAEENEKNLEDQSSKAGD MGNCVSGQQQ EGGVSEEMKG PVQEDKGEQL
• Sequence Length: 578

• Applicable Applications for Raftlin recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.0kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ser288~Leu520
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 41872577
• NCBI GeneID: 23180
• NCBI Accession #: NP_055965.1
• NCBI GenBank Nucleotide #: NM_015150.1
• Molecular Weight: 63,146 Da
• NCBI Official Full Name: raftlin
• NCBI Official Synonym Full Names: raftlin, lipid raft linker 1
• NCBI Official Symbol: RFTN1
• NCBI Official Synonym Symbols: MIG2; PIG9; PIB10; RAFTLIN
• NCBI Protein Information: raftlin
• UniProt Protein Name: Raftlin
• UniProt Gene Name: RFTN1
• UniProt Synonym Gene Names: KIAA0084
• UniProt Entry Name: RFTN1_HUMAN

Uniprot Description

RFTN1: May play a pivotal role in the formation and/or maintenance of lipid rafts. May regulate B-cell antigen receptor- mediated signaling. Belongs to the raftlin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3p24.3

Cellular Component: protein complex; cytoplasm; plasma membrane; endosome; lipid raft

Molecular Function: double-stranded RNA binding

Biological Process: protein transport into lipid raft; B cell receptor signaling pathway; response to exogenous dsRNA; T cell antigen processing and presentation; interleukin-17 production; dsRNA transport; toll-like receptor 3 signaling pathway; lipid raft formation; T cell receptor signaling pathway; growth