Reverse Transcriptase, M-MLV Recombinant Protein
Reverse Transcriptase, M-MLV (M-MLV-RT) Recombinant
Purity
Molecular Biology Grade90%, Verified by SDS-PAGE
Synonyms
Reverse Transcriptase; M-MLV; M-MLV (M-MLV-RT) Recombinant; M-MLV recombinant protein
Host
Recombinant expressed in E Coli
Purity/Purification
Molecular Biology Grade
90%, Verified by SDS-PAGE
90%, Verified by SDS-PAGE
Form/Format
Supplied as a liquid in 20mM Tris HCl, pH 7.5, 200mM sodium chloride, 0.1mM EDTA, 1mM DTT, 0.01% NP-40 and 50% glycerol.
Application Notes
Suitable for use in In Situ Transcription, cDNA Library Synthesis and Screening, mRNA Structure Determination and Ribosomal Pause Site Determination.
Dilution: M-MLV Reverse Transcriptase is less processive than AMV Reverse Transcriptase, and therefore, more units of the M-MLV enzyme are required to generate the same amount of cDNA as in the AMV reaction. Thus, starting with 1µg of mRNA in a first-strand cDNA synthesis, 200 units of the M-MLV enzyme are recommended as opposed to 25 units of the AMV enzyme.
Optimal dilutions to be determined by researcher.
Dilution: M-MLV Reverse Transcriptase is less processive than AMV Reverse Transcriptase, and therefore, more units of the M-MLV enzyme are required to generate the same amount of cDNA as in the AMV reaction. Thus, starting with 1µg of mRNA in a first-strand cDNA synthesis, 200 units of the M-MLV enzyme are recommended as opposed to 25 units of the AMV enzyme.
Optimal dilutions to be determined by researcher.
CAS Number
9068-38-6
Unit Definition
One unit is defined as that amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37 degree C. The reaction conditions are: 50mM Tris-HCl (pH 8.3), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H] dTTP, 0.025mM oligo(dT50), 0.25mM poly(A)400 and 0.01% NP-40.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Reverse Transcriptase, M-MLV recombinant protein
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (> 5kb). M-MLV RT is the preferred reverse transcriptase for long mRNA templates because the RNase H activity of M-MLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Product Categories/Family for Reverse Transcriptase, M-MLV recombinant protein
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Product Notes
The Reverse Transcriptase, M-MLV (Catalog #AAA636119) is a Recombinant Protein produced from Recombinant expressed in E Coli and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in In Situ Transcription, cDNA Library Synthesis and Screening, mRNA Structure Determination and Ribosomal Pause Site Determination. Dilution: M-MLV Reverse Transcriptase is less processive than AMV Reverse Transcriptase, and therefore, more units of the M-MLV enzyme are required to generate the same amount of cDNA as in the AMV reaction. Thus, starting with 1µg of mRNA in a first-strand cDNA synthesis, 200 units of the M-MLV enzyme are recommended as opposed to 25 units of the AMV enzyme. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Reverse Transcriptase, M-MLV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Reverse Transcriptase, M-MLV, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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