Western Blot (WB)
(MAGEL2 Antibody (D514) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEL2 antibody detected the MAGEL2 protein (arrow).)
Rabbit anti-Human MAGEL2 Polyclonal Antibody | anti-MAGEL2 antibody
MAGEL2 Antibody (C-term)
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MAGEL2; Polyclonal Antibody; MAGEL2 Antibody (C-term); MAGE-like protein 2; Necdin-like protein 1; Protein nM15; NDNL1; anti-MAGEL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MAGEL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499-529 amino acids from the C-terminal region of human MAGEL2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.26 (varies by lot)
Sequence Positions
499-529
Sequence Length
529
Applicable Applications for anti-MAGEL2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(MAGEL2 Antibody (D514) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEL2 antibody detected the MAGEL2 protein (arrow).)
Western Blot (WB)
(MAGEL2 Antibody (D514) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEL2 antibody detected the MAGEL2 protein (arrow).)
Immunohistochemistry (IHC)
(MAGEL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MAGEL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-MAGEL2 antibody
Melanoma-associated antigen (MAGE) are completely silent in normal tissues, with the exception of male germ cells, and, for some of them, placenta. These antigens ought to be strictly tumor specific, expressed in tumor cells of various histological types. Because of their specific expression on tumor cells, these antigens are of particular interest for antitumor immunotherapy. Genes of the MAGE family direct the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes. Though the function of MAGE is unknown, may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Product Categories/Family for anti-MAGEL2 antibody
References
Lee, S., et al., Hum. Mol. Genet. 9(12):1813-1819 (2000).
Boccaccio, I., et al., Hum. Mol. Genet. 8(13):2497-2505 (1999).
Boccaccio, I., et al., Hum. Mol. Genet. 8(13):2497-2505 (1999).
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
58669
NCBI Official Full Name
MAGE-like protein 2
UniProt Protein Name
MAGE-like protein 2
Protein Family
UniProt Gene Name
MAGEL2
UniProt Synonym Gene Names
NDNL1
UniProt Entry Name
MAGL2_HUMAN
Uniprot Description
MAGE-L2: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Interacts with TRIM27
Chromosomal Location of Human Ortholog: 15q11.2
Cellular Component: retromer complex; early endosome; nucleus; endosome
Molecular Function: protein binding; ubiquitin-protein ligase activity
Biological Process: transcription, DNA-dependent; rhythmic process; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; retrograde transport, endosome to Golgi
Disease: Prader-willi Syndrome; Prader-willi-like Syndrome
Similar Products
Product Notes
The MAGEL2 magel2 (Catalog #AAA9204334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 499-529. The MAGEL2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGEL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the MAGEL2 magel2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGEL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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