Mouse Resistin ELISA Kit | RETN elisa kit

Mouse Resistin PicoKine ELISA Kit

Cat. Num. AAA175990

• Gene Names: Retn; ADSF; Rstn; Xcp4; Fizz3
• Reactivity: Mouse
• Synonyms: Resistin; Mouse Resistin PicoKine ELISA Kit; resistin; Adipose tissue specific secretory factor; Adipose tissue-specific secretory factor; ADSF; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine rich secreted protein A12 alpha like 2; Cysteine rich secreted protein FIZZ3; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ 3; FIZZ3; Found in inflammatory zone 3; HXCP 1; HXCP1; MGC126603; MGC126609; PRO1199; Resistin delta2; RETN; RETN 1; RETN_HUMAN; RETN1; UNQ407; XCP 1; XCP1; RETN elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Resistin
• Sequence Length: 114

• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 2pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Resistin PicoKine ELISA Kit standard curve )

Related Product Information for RETN elisa kit

Principle of the assay: mouse Resistin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Resistin has been precoated onto 96-well plates. Standards (E.coli,S21-S114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Resistin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Resistin amount of sample captured in plate.
Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 81870648
• NCBI GeneID: 57264
• UniProt Accession #: Q5BMX4; Q99P87
• Molecular Weight: 12,492 Da
• NCBI Official Full Name: Resistin (18-day embryo whole body cDNA, RIKEN full-length enriched library, clone:1110029G18 product:resistin, full insert sequence)
• NCBI Official Synonym Full Names: resistin
• NCBI Official Symbol: Retn
• NCBI Official Synonym Symbols: ADSF; Rstn; Xcp4; Fizz3
• NCBI Protein Information: resistin; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; dominant inhibitory adipocyte-specific secretory factor; adipose-specific cysteine-rich secreted protein A12-alpha
• UniProt Protein Name: Resistin
• Protein Family: Resistin
• UniProt Gene Name: Retn
• UniProt Entry Name: Q5BMX4_MOUSE

Uniprot Description

resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: hormone activity

Biological Process: fat cell differentiation; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of synaptic transmission; response to insulin stimulus