Reelin (RL) Recombinant Protein | RL recombinant protein

Recombinant Reelin (RL)

Cat. Num. AAA2018226

• Gene Names: Reln; rl; reeler
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Reelin (RL); Recombinant Reelin (RL); RL recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- EYTKDARS DSWQLVQTQC LPSSSNSIGC SPFQFHEATI YNAVNSSSWK RITIQLPDHV SSSATQFRWI QKGEETEKQS WAIDHVYIGE ACPKLCSGHG YCTTGAVCIC DESFQGDDCS VFSHELPSYI KDNFESARVT EANWETIQGG VIGSGCGQLA PYAHGDSLYF NGCQIRQAAT KPLDLTRASK IMFVLQIGSP AQTDSCNSDL SGPHTVDKAV LLQYSVNNGI TWHVIAQHQP KDFTQAQRVS YNVPLEARMK GVLLRWWQPR HNGTGHDQWA LDHVEVVLVS TRKQNYMMNF SRQHGLRHFY NRRRRSLRRY P
• Sequence Length: 3461

• Applicable Applications for RL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Glu3143~Pro3461 (Accession # Q60841) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 117320554
• NCBI GeneID: 19699
• NCBI Accession #: NP_035391.2
• NCBI GenBank Nucleotide #: NM_011261.2
• UniProt Accession #: Q60841; Q9CUA6; E9PZ78
• Molecular Weight: 41.8kDa
• NCBI Official Full Name: reelin
• NCBI Official Synonym Full Names: reelin
• NCBI Official Symbol: Reln
• NCBI Official Synonym Symbols: rl; reeler
• NCBI Protein Information: reelin; extracellular matrix serine protease
• UniProt Protein Name: Reelin
• UniProt Gene Name: Reln
• UniProt Synonym Gene Names: Rl
• UniProt Entry Name: RELN_MOUSE

Uniprot Description

reelin: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2); also known as lissencephaly with cerebellar hypoplasia or Norman-Roberts syndrome. LIS2 is a classic type lissencephaly associated with abnormalities of the cerebellum, hippocampus and brainstem. Individuals with LIS2 are severely ataxic, mentally retarded and suffer from epilepsy. Belongs to the reelin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; Cell adhesion; EC 3.4.21.-; Secreted; Secreted, signal peptide; Protease

Cellular Component: extracellular space; proteinaceous extracellular matrix; neuron projection; cell soma; axon; dendrite; cytoplasm; extracellular region; perikaryon

Molecular Function: peptidase activity; protein binding; hydrolase activity; serine-type peptidase activity; metal ion binding

Biological Process: axon guidance; cellular morphogenesis during differentiation; peptidyl-tyrosine phosphorylation; central nervous system development; spinal cord patterning; multicellular organismal development; neuron migration; response to pain; locomotory behavior; proteolysis; positive regulation of synaptic transmission, glutamatergic; glial cell differentiation; forebrain development; dendrite development; cell adhesion; layer formation in the cerebral cortex; associative learning; positive regulation of TOR signaling pathway; thyroid hormone metabolic process; regulation of behavior; regulation of synaptic transmission; cell migration; dentate gyrus development; cerebral cortex tangential migration; hippocampus development; activation of CREB transcription factor; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of small GTPase mediated signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; long-term memory; regulation of gene expression; positive regulation of protein kinase activity; cerebral cortex development; brain development