Human Reelin ELISA Kit | RELN elisa kit

Human Reelin ELISA Kit

Cat. Num. AAA9428141

• Gene Names: RELN; RL; ETL7; LIS2; PRO1598
• Reactivity: Human
• Synonyms: Reelin; Human Reelin ELISA Kit; RELN; 3.4.21.-; RELN elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Reelin. No significant cross-reactivity or interference was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between human Reelin and all the analogues, therefore, cross reaction may still exist.

• Samples: Serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 15.6 pg/mL -1000 pg/mL
• Sensitivity: 8.5 pg/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RELN elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human reelin concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reelin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Reelin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Reelin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Reelin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27436938
• NCBI GeneID: 5649
• NCBI Accession #: NP_005036.2
• NCBI GenBank Nucleotide #: NM_005045.3
• UniProt Accession #: P78509; Q86UJ0; Q86UJ8; Q8NDV0; Q9UDQ2; A4D0P9; A4D0Q0
• Molecular Weight: 384,093 Da
• NCBI Official Full Name: reelin isoform a
• NCBI Official Synonym Full Names: reelin
• NCBI Official Symbol: RELN
• NCBI Official Synonym Symbols: RL; ETL7; LIS2; PRO1598
• NCBI Protein Information: reelin
• UniProt Protein Name: Reelin
• Protein Family: Reelin
• UniProt Gene Name: RELN

NCBI Description

This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

reelin: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2); also known as lissencephaly with cerebellar hypoplasia or Norman-Roberts syndrome. LIS2 is a classic type lissencephaly associated with abnormalities of the cerebellum, hippocampus and brainstem. Individuals with LIS2 are severely ataxic, mentally retarded and suffer from epilepsy. Belongs to the reelin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell development/differentiation; EC 3.4.21.-; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: cytoplasm; dendrite; extracellular region; extracellular space

Molecular Function: protein serine/threonine/tyrosine kinase activity

Biological Process: activation of CREB transcription factor; axon guidance; brain development; cellular morphogenesis during differentiation; central nervous system development; cerebral cortex tangential migration; glial cell differentiation; hippocampus development; neuron migration; peptidyl-tyrosine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase activity; positive regulation of small GTPase mediated signal transduction; positive regulation of synaptic transmission, glutamatergic; regulation of behavior; regulation of synaptic transmission; response to pain; spinal cord patterning

Disease: Epilepsy, Familial Temporal Lobe, 1; Epilepsy, Familial Temporal Lobe, 7; Lissencephaly 2