Human Dihydropyrimidinase (DPYS) ELISA Kit | DPYS elisa kit

Human Dihydropyrimidinase (DPYS) ELISA Kit

Cat. Num. AAA2021818

• Gene Names: DPYS; DHP; DHPase
• Reactivity: Human
• Synonyms: Dihydropyrimidinase (DPYS); Human Dihydropyrimidinase (DPYS) ELISA Kit; DHP; DHPase; 5; 6-Dihydropyrimidine Amidohydrolase; Hydantoinase; DPYS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dihydropyrimidinase (DPYS).; No significant cross-reactivity or interference between Dihydropyrimidinase (DPYS) and analogues was observed.
• Sequence Length: 519

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydropyrimidinase (DPYS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydropyrimidinase (DPYS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPYS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidinase (DPYS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidinase (DPYS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidinase (DPYS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydropyrimidinase (DPYS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DPYS elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4503375
• NCBI GeneID: 1807
• NCBI Accession #: NP_001376.1
• NCBI GenBank Nucleotide #: NM_001385.2
• UniProt Accession #: Q14117
• Molecular Weight: 56,630 Da
• NCBI Official Full Name: dihydropyrimidinase
• NCBI Official Synonym Full Names: dihydropyrimidinase
• NCBI Official Symbol: DPYS
• NCBI Official Synonym Symbols: DHP; DHPase
• NCBI Protein Information: dihydropyrimidinase
• UniProt Protein Name: Dihydropyrimidinase
• UniProt Gene Name: DPYS
• UniProt Synonym Gene Names: DHP; DHPase
• UniProt Entry Name: DPYS_HUMAN

NCBI Description

Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria. [provided by RefSeq, Jul 2008]

Uniprot Description

DPYS: Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6- dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate. Defects in DPYS are the cause of dihydropyrimidinase deficiency (DHPD). DHPD is an autosomal recessive disorder characterized by dihydropyrimidinuria and associated with a variable clinical phenotype: epileptic or convulsive attacks, dysmorphic features and severe developmental delay, and congenital microvillous atrophy. Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 3.5.2.2; Other Amino Acids Metabolism - beta-alanine; Nucleotide Metabolism - pyrimidine; Hydrolase

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: cytosol

Molecular Function: thymine binding; amino acid binding; dihydropyrimidinase activity; zinc ion binding; uracil binding; phosphoprotein binding

Biological Process: beta-alanine metabolic process; pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; uracil catabolic process; thymine catabolic process; pyrimidine base catabolic process; protein homotetramerization

Disease: Dihydropyrimidinase Deficiency