Hyaluronic Acid Binding Protein 1 Active Protein | HABP2 active protein

Hyaluronic Acid Binding Protein 1, Recombinant, Mouse (Hyaluronan Binding Protein 1, HABP1, C1qBP, C1q-R, Human complement component 1, q subcomponent binding protein)

Cat. Num. AAA637529

• Gene Names: HABP2; FSAP; HABP; PHBP; HGFAL
• Purity: Highly Purified; 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/1ug.
• Synonyms: Hyaluronic Acid Binding Protein 1; Recombinant; Mouse (Hyaluronan Binding Protein 1; HABP1; C1qBP; C1q-R; Human complement component 1; q subcomponent binding protein); HABP2 active protein

• Host: Recombinant Mouse
• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/1ug.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

• Activity: Measured by its ability to bind human C1q.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for HABP2 active protein

Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208 aa mature region (3). The 3 kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively. HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6-9). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10-16). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (17).

A DNA sequence encoding the mature mouse HABP1 (Leu 72-Gln 279; Accession # NP_031599), fused with 2X Met at the N-terminus and a six-histidine tag at the C-terminus, was expressed in E. coli.
Calculated molecular mass of ~ 24.8kD.

Product Categories/Family for HABP2 active protein

Molecular Biology; MB-Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 4758502
• NCBI GeneID: 3026
• NCBI Accession #: NP_004123.1
• NCBI GenBank Nucleotide #: NM_004132.3
• UniProt Accession #: Q14520; O00663; A8K467; B7Z8U5; F5H5M6
• Molecular Weight: 62,672 Da
• NCBI Official Full Name: hyaluronan-binding protein 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: hyaluronan binding protein 2
• NCBI Official Symbol: HABP2
• NCBI Official Synonym Symbols: FSAP; HABP; PHBP; HGFAL
• NCBI Protein Information: hyaluronan-binding protein 2; factor VII activating protein; factor VII-activating protease; factor seven-activating protease; hyaluronic acid binding protein 2; plasma hyaluronan binding protein; plasma hyaluronan-binding protein; hepatocyte growth factor activator-like protein
• UniProt Protein Name: Hyaluronan-binding protein 2
• Protein Family: Hyaluronan-binding protein
• UniProt Gene Name: HABP2
• UniProt Synonym Gene Names: HGFAL; PHBP; FSAP
• UniProt Entry Name: HABP2_HUMAN

NCBI Description

The protein encoded by this gene is an extracellular serine protease that binds hyaluronic acid and is involved in cell adhesion. The encoded protein is synthesized as a single chain, but then undergoes an autoproteolytic event to form the functional heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This protease is known to cleave urinary plasminogen activator, coagulation factor VII, and the alpha and beta chains of fibrinogen, but not prothrombin, plasminogen, or the gamma chain of fibrinogen. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

HABP2: Cleaves the alpha-chain at multiple sites and the beta- chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro- urokinase) to the active two chain form. Activates coagulation factor VII. Belongs to the peptidase S1 family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; EC 3.4.21.-; Protease; Cell adhesion

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: extracellular space; extracellular region

Molecular Function: glycosaminoglycan binding; serine-type endopeptidase activity

Biological Process: proteolysis; cell adhesion

Disease: Thrombophilia Due To Thrombin Defect; Thyroid Cancer, Nonmedullary, 5