Mouse HABP ELISA Kit | HABP elisa kit

Mouse HABP (Hya]uronate binding protein) ELISA Kit

Cat. Num. AAA2511190

• Gene Names: HABP2; FSAP; HABP; PHBP; HGFAL
• Reactivity: Mouse
• Synonyms: HABP; Mouse HABP (Hya]uronate binding protein) ELISA Kit; HABP elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse HABP. No significant cross-reactivity or interference between Mouse HABP and analogues was observed.
• Sequence Length: 560

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HABP elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse HABP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HABP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HABP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HABP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HABP. You can calculate the concentration of HABP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758502
• NCBI GeneID: 3026
• NCBI Accession #: NP_004123.1
• NCBI GenBank Nucleotide #: NM_004132.3
• UniProt Accession #: Q14520; O00663; A8K467; B7Z8U5; F5H5M6
• Molecular Weight: 59,864 Da
• NCBI Official Full Name: hyaluronan-binding protein 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: hyaluronan binding protein 2
• NCBI Official Symbol: HABP2
• NCBI Official Synonym Symbols: FSAP; HABP; PHBP; HGFAL
• NCBI Protein Information: hyaluronan-binding protein 2; factor VII activating protein; factor VII-activating protease; factor seven-activating protease; hepatocyte growth factor activator-like protein; hyaluronic acid binding protein 2; plasma hyaluronan binding protein; plasma hyaluronan-binding protein
• UniProt Protein Name: Hyaluronan-binding protein 2
• UniProt Gene Name: HABP2
• UniProt Synonym Gene Names: HGFAL; PHBP; FSAP
• UniProt Entry Name: HABP2_HUMAN

NCBI Description

The protein encoded by this gene is an extracellular serine protease that binds hyaluronic acid and is involved in cell adhesion. The encoded protein is synthesized as a single chain, but then undergoes an autoproteolytic event to form the functional heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This protease is known to cleave urinary plasminogen activator, coagulation factor VII, and the alpha and beta chains of fibrinogen, but not prothrombin, plasminogen, or the gamma chain of fibrinogen. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

HABP2: Cleaves the alpha-chain at multiple sites and the beta- chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro- urokinase) to the active two chain form. Activates coagulation factor VII. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.-; Motility/polarity/chemotaxis; Protease; Secreted; Cell adhesion

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: extracellular space; extracellular region

Molecular Function: glycosaminoglycan binding; serine-type endopeptidase activity

Biological Process: proteolysis; cell adhesion

Disease: Thrombophilia Due To Thrombin Defect; Thyroid Cancer, Nonmedullary, 5