Human hyaluronan binding protein 2 ELISA Kit | HABP2 elisa kit

Human Hyaluronan-binding protein 2, HABP2 ELISA Kit

Cat. Num. AAA906566

• Gene Names: HABP2; FSAP; HABP; PHBP; HGFAL
• Reactivity: Human
• Synonyms: hyaluronan binding protein 2; Human Hyaluronan-binding protein 2; HABP2 ELISA Kit; Human Hyaluronan-binding protein 2 (HABP2) ELISA kit; FSAP; HABP; HGFAL; PHBP; factor VII activating protein; hepatocyte growth factor activator-like protein; hyaluronic acid binding protein 2; plasma hyaluronan binding protein; HABP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human HABP2. No significant cross-reactivity or interference between human HABP2 and analogues was observed.
• Sequence Length: 534

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.312 ng/ml -20 ng/mL
• Sensitivity: <0.078 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HABP2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HABP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HABP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HABP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HABP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 295054188
• NCBI GeneID: 3026
• NCBI Accession #: NP_001171131.1
• NCBI GenBank Nucleotide #: NM_001177660.1
• UniProt Accession #: Q14520; O00663; A8K467; B7Z8U5; F5H5M6
• Molecular Weight: 62,672 Da
• NCBI Official Full Name: hyaluronan-binding protein 2 isoform 2
• NCBI Official Synonym Full Names: hyaluronan binding protein 2
• NCBI Official Symbol: HABP2
• NCBI Official Synonym Symbols: FSAP; HABP; PHBP; HGFAL
• NCBI Protein Information: hyaluronan-binding protein 2; factor VII activating protein; factor VII-activating protease; factor seven-activating protease; hyaluronic acid binding protein 2; plasma hyaluronan binding protein; plasma hyaluronan-binding protein; hepatocyte growth factor activator-like protein
• UniProt Protein Name: Hyaluronan-binding protein 2
• Protein Family: Hyaluronan-binding protein
• UniProt Gene Name: HABP2
• UniProt Synonym Gene Names: HGFAL; PHBP; FSAP
• UniProt Entry Name: HABP2_HUMAN

NCBI Description

The protein encoded by this gene is an extracellular serine protease that binds hyaluronic acid and is involved in cell adhesion. The encoded protein is synthesized as a single chain, but then undergoes an autoproteolytic event to form the functional heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This protease is known to cleave urinary plasminogen activator, coagulation factor VII, and the alpha and beta chains of fibrinogen, but not prothrombin, plasminogen, or the gamma chain of fibrinogen. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

HABP2: Cleaves the alpha-chain at multiple sites and the beta- chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro- urokinase) to the active two chain form. Activates coagulation factor VII. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted, signal peptide; Cell adhesion; Motility/polarity/chemotaxis; Secreted; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: extracellular space; extracellular region

Molecular Function: glycosaminoglycan binding; serine-type endopeptidase activity

Biological Process: proteolysis; cell adhesion

Disease: Thrombophilia Due To Thrombin Defect; Thyroid Cancer, Nonmedullary, 5