Cystatin F Active Protein | CST7 active protein

Cystatin F, Recombinant, Mouse (CMAP, CST7, Cystatin 7, Leukocystatin, Cystatin-like Metastasis-associated Protein)

Cat. Num. AAA636452

• Gene Names: CST7; CMAP
• Purity: Highly Purified; 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Cystatin F; Recombinant; Mouse (CMAP; CST7; Cystatin 7; Leukocystatin; Cystatin-like Metastasis-associated Protein); CST7 active protein

• Host: Recombinant Mouse
• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a liquid in 25mM Tris, 0.15M NaCl, pH 7.5.

• Activity: Measured by its ability to inhibit human cathepsin L.
• Endotoxin Level: 1EU/1ug.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CST7 active protein

Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a new member of the Cystatin superfamily (1-3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards Cathepsin L and legumain (1, 2, 5). Compared to other secreted Cystatins including C, D, E/M, S, SA and SN, which contain two intrachain disulfide bonds, Cystatin F has two extra Cys residues that may be involved in interchain disulfide bonds. rmCystatin F and rhCystatin F exhibit disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human Cystatin F and glutathione S-transferase (2).

Recombinant mouse Cystatin F encoded by the Cst7 gene (rmCystatin F; amino acid residues 19 to 144; Accession # O89098) was expressed with an N-terminal signal sequence and a C-terminal 6X His tag in a murine myeloma cell line, NS0.
Calculated molecular mass of 15kD and migrates as a 20kD band in SDS-PAGE under reducing conditions.

Product Categories/Family for CST7 active protein

Molecular Biology; MB-Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 262263314
• NCBI GeneID: 8530
• NCBI Accession #: NP_003641.3
• NCBI GenBank Nucleotide #: NM_003650.3
• UniProt Accession #: O76096; Q6FH95; Q7Z4J8; Q9UED4
• Molecular Weight: 16,454 Da
• NCBI Official Full Name: cystatin-F
• NCBI Official Synonym Full Names: cystatin F (leukocystatin)
• NCBI Official Symbol: CST7
• NCBI Official Synonym Symbols: CMAP
• NCBI Protein Information: cystatin-F; cystatin-7; leukocystatin; cystatin-like metastasis-associated protein
• UniProt Protein Name: Cystatin-F
• Protein Family: Cystatin
• UniProt Gene Name: CST7
• UniProt Synonym Gene Names: CMAP
• UniProt Entry Name: CYTF_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

CST7: Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system. Belongs to the cystatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cytoplasm; extracellular region

Molecular Function: protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Biological Process: immune response