Rabbit anti-Human, Mouse PPM1L Polyclonal Antibody | anti-PPM1L antibody

PPM1L, CT (PPM1L, PP2CE, Protein phosphatase 1L, Protein phosphatase 1-like, Protein phosphatase 2C isoform epsilon) (APC)

Cat. Num. AAA6336435

• Gene Names: PPM1L; PP2CE; PPM1-LIKE; PP2C-epsilon
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPM1L; Polyclonal Antibody; CT (PPM1L; PP2CE; Protein phosphatase 1L; Protein phosphatase 1-like; Protein phosphatase 2C isoform epsilon) (APC); Protein phosphatase 2C isoform epsilon; anti-PPM1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PPM1L antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PPM1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the C-terminal region of human PPM1L.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PPM1L Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PPM1L antibody detected the PPM1L protein (arrow).)

Immunohistochemistry (IHC)

(PPM1L antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PPM1L antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PPM1L antibody

PPM1L, or PP2CE, belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. PP2Cs regulate stress-activated protein kinase (SAPK; see MIM 601158) signaling cascades that respond to extracellular stimuli (Jin et al., 2004 [PubMed 15560375]).

Product Categories/Family for anti-PPM1L antibody

Antibodies; Enzymes; Phosphatase

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Thean, L.F., et al. Genes Chromosomes Cancer 49(2):99-106(2010). Trynka, G., et al. Gut 58(8):1078-1083(2009). Saito, S., et al. J. Biol. Chem. 283(10):6584-6593(2008). Saito, J., et al. Biochem. J. 405(3):591-596(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 63003905
• NCBI GeneID: 151742
• NCBI Accession #: NP_640338.2
• NCBI GenBank Nucleotide #: NM_139245.2
• UniProt Accession #: Q5SGD2; Q2M3J2; Q96NM7
• Molecular Weight: 41,053 Da
• NCBI Official Full Name: protein phosphatase 1L
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1L
• NCBI Official Symbol: PPM1L
• NCBI Official Synonym Symbols: PP2CE; PPM1-LIKE; PP2C-epsilon
• NCBI Protein Information: protein phosphatase 1L; PP2C epsilon; Protein phosphatase 2C epsilon isoform; protein phosphatase 2C isoform epsilon
• UniProt Protein Name: Protein phosphatase 1L
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1L
• UniProt Synonym Gene Names: PP2CE; PP2C-epsilon
• UniProt Entry Name: PPM1L_HUMAN

NCBI Description

PPM1L, or PP2CE, belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. PP2Cs regulate stress-activated protein kinase (SAPK; see MIM 601158) signaling cascades that respond to extracellular stimuli (Jin et al., 2004 [PubMed 15560375]).[supplied by OMIM, Apr 2008]

Uniprot Description

PPM1L: Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6. Belongs to the PP2C family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 3q26.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: metal ion binding; protein serine/threonine phosphatase activity

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; transmembrane receptor protein serine/threonine kinase signaling pathway; MAPKKK cascade; protein amino acid dephosphorylation