Artemin Active Protein | ARTN active protein

Artemin, Recombinant, Human (GFRalpha3-RET Ligand)

Cat. Num. AAA650419

• Gene Names: ARTN; EVN; NBN; ENOVIN
• Purity: Highly Purified; 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
• Synonyms: Artemin; Recombinant; Human (GFRalpha3-RET Ligand); ARTN active protein

• Host: Recombinant, Human (E Coli)
• Purity/Purification: Highly Purified; 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
• Form/Format: Supplied as a lyophilized powder in 10mM sodium citrate, pH 4.5, 150mM sodium chloride. Reconstitute the lyophilized Human Artemin in sterile ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions.

• Biological Activity: Artemin is fully biologically active when compared to standard. The activity is calculated by it's ability to promote survival and neurite outgrowth and dorsal root ganglion neurons.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm; 2. Analysis by RP-HPLC, using a calibrated solution of Artemin as a Reference Standard.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ARTN active protein

Recombinant Human Artemin is a disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2x113 amino acids.

Product Categories/Family for ARTN active protein

Growth Factors; Cytokines; Growth Factors-Artemin

NCBI and Uniprot Product Information

• NCBI GI #: 4235100
• NCBI GeneID: 9048
• UniProt Accession #: Q5T4W7; O95441; O96030; Q6P6A3; D3DPY1; D3DPY3
• Molecular Weight: 24.2kD
• NCBI Official Full Name: Artemin
• NCBI Official Synonym Full Names: artemin
• NCBI Official Symbol: ARTN
• NCBI Official Synonym Symbols: EVN; NBN; ENOVIN
• NCBI Protein Information: artemin; neublastin; OTTHUMP00000009173; OTTHUMP00000231148; OTTHUMP00000231149; OTTHUMP00000231150; OTTHUMP00000231151; OTTHUMP00000231154; neurotrophic factor
• UniProt Protein Name: Artemin
• Protein Family: Artemin
• UniProt Gene Name: ARTN
• UniProt Synonym Gene Names: EVN
• UniProt Entry Name: ARTN_HUMAN

NCBI Description

The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenerative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

Function: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Ref.1 Ref.2

Subunit structure: Homodimer; disulfide-linked

By similarity. Ref.8

Subcellular location: Secreted

Tissue specificity: Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain. Ref.1 Ref.2

Developmental stage: Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain. Ref.1

Sequence similarities: Belongs to the TGF-beta family. GDNF subfamily.