Nerve Growth Factor, beta Recombinant Active Protein | NGF active protein

Nerve Growth Factor, beta Recombinant, Human (NGFb)

Cat. Num. AAA651016

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF; MGC161426; MGC161428
• Purity: Highly Purified; 98% by RP-HPLC and SDS-PAGE
• Synonyms: Nerve Growth Factor; beta Recombinant; Human (NGFb); NGF active protein

• Host: Recombinant, Human (CHO cells)
• Purity/Purification: Highly Purified; 98% by RP-HPLC and SDS-PAGE
• Form/Format: Supplied as a lyophilized powder from 20mM Tris, 0.15M sodium chloride, pH 8. Reconstitute with sterile, ddH2O to a concentration of 0.1mg/ml.

• Biological Activity: beta-Nerve Growth Factor is fully biologically active when compared to standards. The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be <1.0ng/ml, corresponding to a specific activity of >1x10e6 units/mg.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm using the absorbency value of 1.1945 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant beta-Nerve Growth Factor as a Reference Standard.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for NGF active protein

NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Recombinant protein corresonding to Human Nerve Growth Factor beta produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 118 amino acids. (26.5kD)

Product Categories/Family for NGF active protein

Growth Factors; Cytokines; Growth Factors-NGF

NCBI and Uniprot Product Information

• NCBI GI #: 189203
• NCBI GeneID: 4803
• UniProt Accession #: P01138; Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5
• Molecular Weight: 26,959 Da
• NCBI Official Full Name: nerve growth factor beta
• NCBI Official Synonym Full Names: nerve growth factor (beta polypeptide)
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF; MGC161426; MGC161428
• NCBI Protein Information: beta-nerve growth factor; OTTHUMP00000013653; nerve growth factor, beta subunit
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB
• UniProt Entry Name: NGF_HUMAN

NCBI Description

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq]

Uniprot Description

Function: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Involvement in disease: Defects in NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) [

MIM:608654]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable. Ref.17

Sequence similarities: Belongs to the NGF-beta family.

Sequence caution: The sequence AAH32517.2 differs from that shown. Reason: Erroneous initiation.