Ficolin 1 Active Protein | FCN1 active protein

Ficolin 1, Recombinant, Human (Ficolin-M)

Cat. Num. AAA638134

• Gene Names: FCN1; FCNM
• Reactivity: Human
• Applications: ELISA
• Purity: > 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Ficolin 1; Recombinant; Human (Ficolin-M); FCN1 active protein

• Host: Recombinant, Human CD33 Signal Peptide
• Reactivity: Human
• Specificity: Species sequence homology
• Purity/Purification: > 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to a concentration of 0.1mg/ml.
• Concentration: ~0.1mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for FCN1 active protein: ELISA (EL/EIA)
• Immunogen: NS0-derived recombinant human Ficolin-1.
• Endotoxin: < 1.0 EU per 1ug of the protein as determined by the LAL method.
• Activity: Measured by its ability to bind immobilized Fetuin in a functional ELISA (Liu, Y. et al. (2005) J. Immunol. 175:3150).
• Preparation and Storage: Lyophilized powder is stable for 12 months at -20 degree C Reconstituted product is stable for 3 months at -20 degree C or lower under sterile conditions. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for FCN1 active protein

Mouse myeloma NSO cell line-derived recombinant human Ficolin-1 (Gln28 thru Ala326, NCBI accession #O00602) with a C-terminal 10x His tag.

Ficolin-1 (fibrinogen/collagen-like), also called M-ficolin, is a member of the ficolin family of secreted pattern recognition proteins in the lectin complement activation pathway (1-3). Ficolin-1 is expressed by monocytes, neutrophils and type II alveolar epithelial cells (2-5). It is proposed to be a locally-acting lectin released in a regulated manner (4). Ficolin-1 is not found in plasma, but is detected on the surface of circulating monocytes (4-6). The 35kD, 326 amino acid (aa) human ficolin-1 contains a 28 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like (FBG) domain that includes a calcium binding site and one potential N-glycosylation site. Both the collagen and FBG domains mediate trimer formation (7). Like ficolin-2, larger homo-multimers of ficolin-1 exist and are likely formed by disulfide bonds at the N-terminus. Sizes corresponding to 12 or 18 subunit oligomers are reported (4, 6, 7). The FBG domain of Ficolin-1 binds microbial ligands that contain acetylated compounds (6). Ligands identified include N-acetyl glucosamine, N-acetyl galactosamine and sialyl-N-acetyllactosamine (4-7). Like other ficolins, ficolin-1 associates with, and activates the MBL-associated serine protease (MASP) complex, which activates the complement pathway by cleaving C4, contributing to the innate immune response (4-6). The ficolin-1 gene is polymorphic, showing at least ten single nucleotide polymorphisms in the promoter and coding regions (1).

Product Categories/Family for FCN1 active protein

Molecular Biology; MB-Proteins

References

1. Endo, Y. et al., 2006, Adv. Exp. Med. Biol. 586:265.
2. Endo, Y. et al., 1996, Genomics 36:515. Scand. J. Immunol. 62:462.
3. Lu, J. et al., 1996, Biochem. J. 313: 473.
4. Liu, Y. et al., 2005, J. Immunol. 175:3150.
5. . Teh, C. et al., 2000, Immunology 101:225.
6. Frederiksen, P. D. et al., 2005,
7. Tanio, M. et al., 2007, J. Biol. Chem. 282:3889.
8. Endo, Y. et al., 2004, Genomics 84:737.

NCBI and Uniprot Product Information

• NCBI GI #: 8051584
• NCBI GeneID: 2219
• NCBI Accession #: NP_001994.2
• NCBI GenBank Nucleotide #: NM_002003.3
• UniProt Accession #: O00602; Q5VYV5; Q92596
• Molecular Weight: Predicted: 33.4kD; Observed: 35-36kD (reducing conditions)
• NCBI Official Full Name: ficolin-1
• NCBI Official Synonym Full Names: ficolin (collagen/fibrinogen domain containing) 1
• NCBI Official Symbol: FCN1
• NCBI Official Synonym Symbols: FCNM
• NCBI Protein Information: ficolin-1; M-ficolin; ficolin-A; ficolin-alpha; collagen/fibrinogen domain-containing protein 1; ficolin (collagen/fibrinogen domain-containing) 1
• UniProt Protein Name: Ficolin-1
• Protein Family: Ficolin
• UniProt Gene Name: FCN1
• UniProt Synonym Gene Names: FCNM
• UniProt Entry Name: FCN1_HUMAN

NCBI Description

The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq, Jul 2008]

Uniprot Description

FCN1: Complement-activating lectin and pattern recognition receptor. Binds GlcNAc. Binds preferentially to 9-O-acetylated 2- 6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Belongs to the ficolin lectin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extrinsic to external side of plasma membrane; collagen; extracellular region

Molecular Function: protein binding; G-protein-coupled receptor binding; metal ion binding; carbohydrate binding; pattern recognition receptor activity; sialic acid binding

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of virion penetration into host cell; cell surface pattern recognition receptor signaling pathway; innate immune response; complement activation, lectin pathway; recognition of apoptotic cell; complement activation