Testing Data
Human Ficolin-1/FCN1/M Ficolin ELISA Kit | FCN1 elisa kit
Human Ficolin-1/FCN1/M Ficolin Quick ELISA Kit
O.D.|0.003|0.177|0.313|0.558|0.926|1.491|1.972|2.357
n|16|16|16|
Mean(pg/ml)|1450|8641|19842|
Standard deviation|92.8|475.26|1111.15|
CV(%)|6.4%|5.5%|5.6%|
n|24|24|24
Mean(pg/ml)|1321|8694|19651
Standard deviation|93.79|512.95|1061.15
CV(%)|7.1%|5.9%|5.4%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 1450|1365|1605|1564|1496|97.24|6.5%
Sample 2: 8641|7641|7712|7952|7987|263.55|3.3%
Sample 3: 19842|22647|21642|20458|21147|1141.95|5.4%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Human FCN1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FCN1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FCN1 in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq, Jul 2008]
Uniprot Description
FCN1: Complement-activating lectin and pattern recognition receptor. Binds GlcNAc. Binds preferentially to 9-O-acetylated 2- 6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Belongs to the ficolin lectin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: collagen; extrinsic to external side of plasma membrane; extracellular region
Molecular Function: protein binding; G-protein-coupled receptor binding; metal ion binding; carbohydrate binding; pattern recognition receptor activity; sialic acid binding
Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of virion penetration into host cell; cell surface pattern recognition receptor signaling pathway; innate immune response; complement activation, lectin pathway; recognition of apoptotic cell; complement activation
Research Articles on FCN1
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Product Notes
The Human FCN1 fcn1 (Catalog #AAA1753950) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753950 ELISA Kit recognizes Human FCN1. It is sometimes possible for the material contained within the vial of "Ficolin-1/FCN1/M Ficolin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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