Vascular Endothelial Growth Factor-121, aa207-327 Active Protein
Vascular Endothelial Growth Factor-121, aa207-327, Recombinant, Human (VEGF-121) (BSA Free)
Purity
Highly Purified95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
Vascular Endothelial Growth Factor-121; aa207-327; Recombinant; Human (VEGF-121) (BSA Free); aa207-327 active protein
Host
Recombinant Human
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in 30% CH3CN and 0.1% TFA. Reconstitute with PBS to 100ug/ml
Activity
Measured by its ability to stimulate proliferation in HUVE cells.
Endotoxin Level
1EU/1ug.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Vascular Endothelial Growth Factor-121, aa207-327 active protein
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). VEGF121 is the only form that lacks a basic heparin-binding region and is freely diffusible (4). Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis (5). Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 binds the semaphorin receptor, Neuropilin-1; VEGF121 binding has also been reported (6). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (7, 8). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (9). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-a (3, 4, 10).
A DNA sequence encoding the mature 121 amino acid residues of human VEGF (Ala 207-Arg 327; Accession # NP_001020541) was expressed in E. coli.
Calculated molecular mass of ~ 14kD.
A DNA sequence encoding the mature 121 amino acid residues of human VEGF (Ala 207-Arg 327; Accession # NP_001020541) was expressed in E. coli.
Calculated molecular mass of ~ 14kD.
Product Categories/Family for Vascular Endothelial Growth Factor-121, aa207-327 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
vascular endothelial growth factor 121
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Product Notes
The Vascular Endothelial Growth Factor-121, aa207-327 (Catalog #AAA635981) is an Active Protein produced from Recombinant Human and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Vascular Endothelial Growth Factor-121, aa207-327, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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