Slitrk5 active protein
SLITRK5, Recombinant, Human (Slit and Trk-like Family Member 5, LRR-containing Protein 11)
Gene Names
Slitrk5; 2610019D03Rik
Purity
Highly Purified95%, as determined by SDS-PAGE and visualized by silver stain.
Synonyms
SLITRK5; Recombinant; Human (Slit and Trk-like Family Member 5; LRR-containing Protein 11); Slitrk5 active protein
Host
Recombinant Human
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE and visualized by silver stain.
95%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.
Activity
Measured by its ability to induce neurite outgrowth of E13 chick dorsal root ganglia neurons.
Endotoxin Level
1.0 EU/1 ug.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Slitrk5 active protein
SLITRK5 (Slit and Trk-like family member 5; also LRR-containing protein 11) is a member of the SLITRK family of proteins.1, 2 This family currently includes six members, all of which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region.3 Human SLITRK5 is a type I transmembrane (TM) protein that is 958 amino acids (aa) in length. It contains a putative 40 aa signal sequence, a 624 aa extracellular domain (ECD), a 21 aa TM segment, and a 273 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa # 80-285 plus aa # 384-579) contains seven and eight leucine-rich repeats (LRRs), respectively. The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction.4 The cytoplasmic region contains two conserved, potential phosphorylation sites at Tyr 833 and Tyr 917, plus a TrkA-like tyrosine phosphorylation site (YLxxL) at Tyr 945. SLITRK5 is expressed on embryonic neurons of the hippocampus (pyramidal layer) and thalamus.3 Based on SLITRK2 studies, SLITRK5 will block neurite extension.3 Human SLITRK5 shares 97% and 98% aa identity with mouse and canine SLITRK5, respectively.
A DNA sequence encoding the extracellular domain of human SLITRK5 (Glu 39-Val 665; Accession # NP_056382) was fused with a 6X histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of ~ 70.8kD. As a result of glycosylation, recombinant human SLITRK5 migrates as an ~ 100-120kD protein in SDS-PAGE under reducing conditions.
A DNA sequence encoding the extracellular domain of human SLITRK5 (Glu 39-Val 665; Accession # NP_056382) was fused with a 6X histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of ~ 70.8kD. As a result of glycosylation, recombinant human SLITRK5 migrates as an ~ 100-120kD protein in SDS-PAGE under reducing conditions.
Product Categories/Family for Slitrk5 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,165 Da
NCBI Official Full Name
SLIT and NTRK-like protein 5
NCBI Official Synonym Full Names
SLIT and NTRK-like family, member 5
NCBI Official Symbol
Slitrk5
NCBI Official Synonym Symbols
2610019D03Rik
NCBI Protein Information
SLIT and NTRK-like protein 5; slit and trk like gene 5
UniProt Protein Name
SLIT and NTRK-like protein 5
Protein Family
UniProt Gene Name
Slitrk5
UniProt Entry Name
SLIK5_MOUSE
Uniprot Description
SLITRK5: Suppresses neurite outgrowth. Belongs to the SLITRK family.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; receptor complex
Biological Process: skin development; grooming behavior; response to xenobiotic stimulus; synaptic transmission; striatum development; axonogenesis; adult behavior; dendrite morphogenesis
Research Articles on Slitrk5
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Product Notes
The Slitrk5 slitrk5 (Catalog #AAA637453) is an Active Protein produced from Recombinant Human and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SLITRK5, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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