Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
TLK2 recombinant protein
TLK2, Active
Applications
Western Blot
Synonyms
TLK2; Active; MGC44450; PKU-ALPHA; TLK2 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for TLK2 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for TLK2 recombinant protein
Recombinant human TLK2 (388-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_006852.
TLK2 or Tousled-like kinase 2, is a nuclear serine/threonine kinases that is thought to be involved in the regulation of chromatin assembly (1). In addition, TLK2 is also involved in other cellular processes such as intracellular signaling cascade, protein amino acid phosphorylation, cell cycle, chromatin modification and in response to DNA damage stimulus. TLK2 displays maximal activity during S phase of the cell cycle where it appears to be regulated by cell-cycle-dependent phosphorylation. Inhibition of DNA replication causes a rapid loss of TLK2 activity, indicating that TLK2 function is tightly linked to ongoing DNA replication.
TLK2 or Tousled-like kinase 2, is a nuclear serine/threonine kinases that is thought to be involved in the regulation of chromatin assembly (1). In addition, TLK2 is also involved in other cellular processes such as intracellular signaling cascade, protein amino acid phosphorylation, cell cycle, chromatin modification and in response to DNA damage stimulus. TLK2 displays maximal activity during S phase of the cell cycle where it appears to be regulated by cell-cycle-dependent phosphorylation. Inhibition of DNA replication causes a rapid loss of TLK2 activity, indicating that TLK2 function is tightly linked to ongoing DNA replication.
Product Categories/Family for TLK2 recombinant protein
References
1.Yamakawa, A. et al: cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal. Gene,1998; 202 (1-2): 193-201.
2.Sillje, H. H. W. et al: Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication. EMBO J. 18: 5691-5702, 1999.
2.Sillje, H. H. W. et al: Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication. EMBO J. 18: 5691-5702, 1999.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~67kDa
Protein Family
Similar Products
Product Notes
The TLK2 (Catalog #AAA517691) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TLK2 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the TLK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLK2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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