Testing Data
(Fig. The sample is Mouse TNF-alpha protein (PRP1113, 17KD), the primary antibody is TNF-alpha Polyclonal Antibody (ABP0127, 1:2500), and the secondary antibody is HRP, Goat Anti-rabbit IgG (1:10000). The exposure time is 30s.)
West Pico PLUS Chemiluminescent Substrate Reagent
SuperKine West Pico PLUS Chemiluminescent Substrate
Synonyms
West Pico PLUS Chemiluminescent Substrate; SuperKine West Pico PLUS Chemiluminescent Substrate; Enhanced Chemiluminescence; ECL; Chemiluminescence; Electrochemiluminescence; West Pico PLUS Chemiluminescent Substrate reagent
Tag
ECL
Features & Benefits
The SuperKine West Pico PLUS Chemiluminescent Substrate developed by is based on the innovation and optimization of the classic peroxidase substrate system. It is used for enhanced chemiluminescence (ECL) and can directly replace other expensive ECL products without the need to renew Optimize experimental conditions.
Components
ECL HRP Substrate Reagent A
ECL HRP Substrate Reagent B
SuperKine West Pico PLUS Chemiluminescent Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments. In order to obtain the best experimental results, you need to optimize all your experimental factors, including the number of samples, antibody concentration, the use of membranes and blocking agents.
ECL HRP Substrate Reagent B
SuperKine West Pico PLUS Chemiluminescent Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments. In order to obtain the best experimental results, you need to optimize all your experimental factors, including the number of samples, antibody concentration, the use of membranes and blocking agents.
Usage Notes
Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution.
Suggest 0.1-0.2ml of SuperLumia ECL working solution for 1 cm2 size of blot membrane.
Incubate blot 1-5 minutes in SuperLumia ECL Substrate working solution.
Drain excess reagent. Cover blot with clear plastic wrap.
Expose blot to X-ray film.
Suggest 0.1-0.2ml of SuperLumia ECL working solution for 1 cm2 size of blot membrane.
Incubate blot 1-5 minutes in SuperLumia ECL Substrate working solution.
Drain excess reagent. Cover blot with clear plastic wrap.
Expose blot to X-ray film.
Preparation and Storage
Stable for one year at 4-8 degree C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Testing Data
(Fig. The sample is Mouse TNF-alpha protein (PRP1113, 17KD), the primary antibody is TNF-alpha Polyclonal Antibody (ABP0127, 1:2500), and the secondary antibody is HRP, Goat Anti-rabbit IgG (1:10000). The exposure time is 30s.)
Testing Data
(Fig. The sample is Mouse TNF-alpha protein (PRP1113, 17KD), the primary antibody is TNF-alpha Polyclonal Antibody (ABP0127, 1:2500), and the secondary antibody is HRP, Goat Anti-rabbit IgG (1:10000). The exposure time is 30s.)
Related Product Information for West Pico PLUS Chemiluminescent Substrate reagent
Description: ECL
Background: The family of ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. The SuperKine West Pico PLUS Chemiluminescent Substrate is specially optimized for picogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate's intense signal output and duration enables detection of picogram amounts of antigen with photographic or other imaging methods perfectly well.
Background: The family of ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. The SuperKine West Pico PLUS Chemiluminescent Substrate is specially optimized for picogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate's intense signal output and duration enables detection of picogram amounts of antigen with photographic or other imaging methods perfectly well.
Product Categories/Family for West Pico PLUS Chemiluminescent Substrate reagent
Similar Products
Product Notes
The West Pico PLUS Chemiluminescent Substrate (Catalog #AAA9719194) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "West Pico PLUS Chemiluminescent Substrate, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
