Human Arsenic, 3-Oxidation State Methyltransferase ELISA Kit | AS3MT elisa kit

Human Arsenic, 3-Oxidation State Methyltransferase (AS3MT) ELISA Kit

Cat. Num. AAA2031857

• Gene Names: AS3MT; CYT19
• Reactivity: Human
• Synonyms: Arsenic; 3-Oxidation State Methyltransferase; Human Arsenic; 3-Oxidation State Methyltransferase (AS3MT) ELISA Kit; CYT19; Arsenite Methyltransferase; Methylarsonite methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase; AS3MT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arsenic, 3-Oxidation State Methyltransferase (AS3MT).; No significant cross-reactivity or interference between Arsenic, 3-Oxidation State Methyltransferase (AS3MT) and analogues was observed.
• Sequence Length: 375

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.114ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arsenic, 3-Oxidation State Methyltransferase (AS3MT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arsenic, 3-Oxidation State Methyltransferase (AS3MT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for AS3MT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arsenic, 3-Oxidation State Methyltransferase (AS3MT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arsenic, 3-Oxidation State Methyltransferase (AS3MT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arsenic, 3-Oxidation State Methyltransferase (AS3MT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arsenic, 3-Oxidation State Methyltransferase (AS3MT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AS3MT elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 55925944
• NCBI GeneID: 57412
• Molecular Weight: 31,098 Da
• NCBI Official Full Name: arsenic (+3 oxidation state) methyltransferase
• NCBI Official Synonym Full Names: arsenite methyltransferase
• NCBI Official Symbol: AS3MT
• NCBI Official Synonym Symbols: CYT19
• NCBI Protein Information: arsenite methyltransferase
• UniProt Protein Name: Arsenite methyltransferase
• Protein Family: Arsenite methyltransferase
• UniProt Gene Name: AS3MT
• UniProt Synonym Gene Names: CYT19
• UniProt Entry Name: AS3MT_HUMAN

NCBI Description

AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM, Mar 2008]

Uniprot Description

AS3MT: Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me- AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH. Belongs to the methyltransferase superfamily.

Protein type: Methyltransferase; Mitochondrial; EC 2.1.1.137

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: cytosol; mitochondrion

Molecular Function: arsenite methyltransferase activity; methylarsonite methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity

Biological Process: arsonoacetate metabolic process; methylation; toxin metabolic process; xenobiotic metabolic process