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Plant Genomic DNA Reagent
Plant Genomic DNA Isolation Reagent (Reagent Based)
Synonyms
Plant Genomic DNA; Plant Genomic DNA Isolation Reagent (Reagent Based); Plant Genomic DNA reagent
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Related Product Information for Plant Genomic DNA reagent
Principle of the Assay: The PG Reagent provides an easy 3-step method to isolate a high yield of total DNA (including genomic, mitochondrial, and chloroplast DNA from the plant tissue and cells). This unique reagent is able to lyse the most common plant samples as well as the samples high in polysaccharides. If a large sample is required, the reagent volume can be scaled proportionately, making this reagent not only very user-friendly but also highly versatile. The DNA phenol extraction is not required, and the entire procedure can be completed in 90 minutes. The extracted total DNA is ready for use in PCR, Real-time PCR, Southern Blotting, Mapping and RFLP.
Background/Introduction: The goal of the genomic DNA isolation depends on the type(s) of DNA application after isolation. The DNA's purity, source, quantity, and quality are all key issues that need to be addressed prior to the genomic DNA extraction. A whole host of different methods, technologies and kits are available now to researchers to isolate the genomic DNA from cells. Generally, all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA by RNAse, salt or other methods. The DNA is isolated from the proteins by several methods, including the digestion of proteins by the enzyme proteinase K. The proteins are removed subsequently by salting-out, organic extraction, or binding of the DNA to a solid-phase support (such as an anion-exchange column or silica technology). The DNA is finally recovered by ethanol precipitation or isopropanol precipitation.
Background/Introduction: The goal of the genomic DNA isolation depends on the type(s) of DNA application after isolation. The DNA's purity, source, quantity, and quality are all key issues that need to be addressed prior to the genomic DNA extraction. A whole host of different methods, technologies and kits are available now to researchers to isolate the genomic DNA from cells. Generally, all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA by RNAse, salt or other methods. The DNA is isolated from the proteins by several methods, including the digestion of proteins by the enzyme proteinase K. The proteins are removed subsequently by salting-out, organic extraction, or binding of the DNA to a solid-phase support (such as an anion-exchange column or silica technology). The DNA is finally recovered by ethanol precipitation or isopropanol precipitation.
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Product Notes
The Plant Genomic DNA (Catalog #AAA402051) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Plant Genomic DNA, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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