Rabbit anti-Human DDX56 Polyclonal Antibody | anti-DDX56 antibody

DDX56, CT (DDX56, DDX21, NOH61, Probable ATP-dependent RNA helicase DDX56, ATP-dependent 61kD nucleolar RNA helicase, DEAD box protein 21, DEAD box protein 56) (FITC)

Cat. Num. AAA6291522

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX56; Polyclonal Antibody; CT (DDX56; DDX21; NOH61; Probable ATP-dependent RNA helicase DDX56; ATP-dependent 61kD nucleolar RNA helicase; DEAD box protein 21; DEAD box protein 56) (FITC); DEAD box protein 56; anti-DDX56 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DDX56 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DDX56 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-546 amino acids from the C-terminal region of human DDX56.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DDX56 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the DDX56 antibody detected the DDX56 protein (arrow).)

Related Product Information for anti-DDX56 antibody

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit.

Product Categories/Family for anti-DDX56 antibody

Antibodies; DEAD Box Proteins

References

Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Andersen, J.S., et al. Nature 433(7021):77-83(2005). Lehner, B., et al. Genome Res. 14(7):1315-1323(2004). Scherl, A., et al. Mol. Biol. Cell 13(11):4100-4109(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 9506931
• NCBI Accession #: NP_061955.1
• NCBI GenBank Nucleotide #: NM_019082.3
• UniProt Accession #: Q9NY93
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX56 isoform 1
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX56
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX56
• UniProt Synonym Gene Names: DDX21; NOH61
• UniProt Entry Name: DDX56_HUMAN

Uniprot Description

DDX56: May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity. Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.

Protein type: Nucleolus; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: membrane; nucleolus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity

Biological Process: rRNA processing