HRP Conjugate Stock Stabilizing Reagent
HRP Conjugate Stock Stabilizing Reagent, 5X
Synonyms
HRP Conjugate Stock Stabilizing Reagent; 5X; HRP Conjugate Stock Stabilizer; HRP Conjugate Stock Stabilizing Reagent reagent
Form/Format
Supplied at 5X.
Application Notes
When using HRP Conjugate Stock Stabilizer, 5X as a Conjugate Diluent:
1. Add 1 part HRP Conjugate Stock Stabilizer, 5X to 4 parts deionized water and mix for 5-15 minutes.
2. Add the antibody-HRP or protein-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes.
3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8 degree C for use at a later date. 1X working conjugate dilutions can be stored at 2-8 degree C, protected from light, providing a useful shelf-life of 6-12 months.
When using HRP Conjugate Stock Stabilizer, 5X as a Concentrated Conjugate Stock Storage Buffer:
1. To purified HRP conjugate, add an equal volume of the 5X HRP Conjugate Stock Stabilizer.
2. Filter sterilize into a sterile amber or brown-glass container.
3. Store at 2-8 degree C. Concentrated HRP conjugate stocks should be stable for up to 18 months.
1. Add 1 part HRP Conjugate Stock Stabilizer, 5X to 4 parts deionized water and mix for 5-15 minutes.
2. Add the antibody-HRP or protein-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes.
3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8 degree C for use at a later date. 1X working conjugate dilutions can be stored at 2-8 degree C, protected from light, providing a useful shelf-life of 6-12 months.
When using HRP Conjugate Stock Stabilizer, 5X as a Concentrated Conjugate Stock Storage Buffer:
1. To purified HRP conjugate, add an equal volume of the 5X HRP Conjugate Stock Stabilizer.
2. Filter sterilize into a sterile amber or brown-glass container.
3. Store at 2-8 degree C. Concentrated HRP conjugate stocks should be stable for up to 18 months.
pH
7.4 at 1X
Contains
Mammalian Proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for HRP Conjugate Stock Stabilizing Reagent reagent
Description: HRP Conjugate Stock Stabilizer is best for users who desire an all-purpose HRP conjugate diluent and HRP stabilizer. This formulation may serve as a base to add specialized components to address non-specific binding and interference. It also serves as an excellent concentrated buffer to store HRP conjugates.
Background: HRP Conjugate Stock Stabilizer, 5X contains a mammalian protein additive to stabilize reconstituted HRP-conjugated proteins, as well as stabilizers to preserve the catalytic site of the HRP porphyrin ring structure and strengthen the HRP-IgG bond. This product is best for use as a conjugate stabilizing diluent, conjugate stabilizer base reagent, and as a concentrated conjugated stock storage buffer. Many find this product particularly useful for stabilizing in-house conjugations for long term storage. Our conjugate stabilizers contain antimicrobial agents have been added to retard bacterial growth.
Background: HRP Conjugate Stock Stabilizer, 5X contains a mammalian protein additive to stabilize reconstituted HRP-conjugated proteins, as well as stabilizers to preserve the catalytic site of the HRP porphyrin ring structure and strengthen the HRP-IgG bond. This product is best for use as a conjugate stabilizing diluent, conjugate stabilizer base reagent, and as a concentrated conjugated stock storage buffer. Many find this product particularly useful for stabilizing in-house conjugations for long term storage. Our conjugate stabilizers contain antimicrobial agents have been added to retard bacterial growth.
Product Categories/Family for HRP Conjugate Stock Stabilizing Reagent reagent
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Product Notes
The HRP Conjugate Stock Stabilizing Reagent (Catalog #AAA258383) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. When using HRP Conjugate Stock Stabilizer, 5X as a Conjugate Diluent: 1. Add 1 part HRP Conjugate Stock Stabilizer, 5X to 4 parts deionized water and mix for 5-15 minutes. 2. Add the antibody-HRP or protein-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes. 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8 degree C for use at a later date. 1X working conjugate dilutions can be stored at 2-8 degree C, protected from light, providing an useful shelf-life of 6-12 months. When using HRP Conjugate Stock Stabilizer, 5X as a Concentrated Conjugate Stock Storage Buffer: 1. To purified HRP conjugate, add an equal volume of the 5X HRP Conjugate Stock Stabilizer. 2. Filter sterilize into a sterile amber or brown-glass container. 3. Store at 2-8 degree C. Concentrated HRP conjugate stocks should be stable for up to 18 months. Researchers should empirically determine the suitability of the HRP Conjugate Stock Stabilizing Reagent for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRP Conjugate Stock Stabilizing Reagent, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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