DAPI blue fluorescent nuclear DNA dye Reagent
DAPI blue fluorescent nuclear DNA dye
Applications
Flow Cytometer, Fluorescence Microscope
Synonyms
DAPI blue fluorescent nuclear DNA dye; DAPI blue fluorescent nuclear DNA dye reagent
Applicable Applications for DAPI blue fluorescent nuclear DNA dye reagent
Flow Cytometer, Fluorescence Microscope
Application Notes
1. To make a 5 mg/mL DAPI stock solution (14.3 mM), dissolve the contents of one vial (10 mg) in 2 mL of deionized water or dimethylformamide (DMF). Note: DAPI is not very soluble in phosphate-buffered saline (PBS).
2. For long term storage, the stock solution can be aliquoted and stored at -20 degree C. For short term storage, the solution can be kept at 2-6 degree C, protected from light. When handled properly, DAPI solutions are stable for at least six months.
3. Dilute the DAPI stock solution to 3 uM in staining buffer. A 1 mL volume will be required for each cell sample.
4. Centrifuge the cell suspension and discard the supernatant. Tap to loosen the pellet and add 1 mL of DAPI diluted in staining buffer.
5. Incubate for 15 minutes at room temperature.
6. Analyze by flow cytometry in the presence of the dye. If the cells are to be viewed by fluorescence microscopy, centrifuge the sample, remove the supernatant and resuspend cells in fresh buffer. Apply a drop of the suspension to a microscope slide, cover with a coverslip and view.
For sample preparation protocols for adherent cells and suspension cells, please see the DAPI product datasheet.
2. For long term storage, the stock solution can be aliquoted and stored at -20 degree C. For short term storage, the solution can be kept at 2-6 degree C, protected from light. When handled properly, DAPI solutions are stable for at least six months.
3. Dilute the DAPI stock solution to 3 uM in staining buffer. A 1 mL volume will be required for each cell sample.
4. Centrifuge the cell suspension and discard the supernatant. Tap to loosen the pellet and add 1 mL of DAPI diluted in staining buffer.
5. Incubate for 15 minutes at room temperature.
6. Analyze by flow cytometry in the presence of the dye. If the cells are to be viewed by fluorescence microscopy, centrifuge the sample, remove the supernatant and resuspend cells in fresh buffer. Apply a drop of the suspension to a microscope slide, cover with a coverslip and view.
For sample preparation protocols for adherent cells and suspension cells, please see the DAPI product datasheet.
Excitation/Emission
358 nm / 461 nm
Preparation and Storage
Store at -20 degree C.
Related Product Information for DAPI blue fluorescent nuclear DNA dye reagent
Background: DAPI is a blue fluorescent DNA stain that forms complexes with natural double-stranded DNA. Use it to detect DNA and visualize nuclear morphology.
Product Categories/Family for DAPI blue fluorescent nuclear DNA dye reagent
Similar Products
Product Notes
The DAPI blue fluorescent nuclear DNA dye (Catalog #AAA258073) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DAPI blue fluorescent nuclear DNA dye can be used in a range of immunoassay formats including, but not limited to, Flow Cytometer, Fluorescence Microscope. 1. To make a 5 mg/mL DAPI stock solution (14.3 mM), dissolve the contents of one vial (10 mg) in 2 mL of deionized water or dimethylformamide (DMF). Note: DAPI is not very soluble in phosphate-buffered saline (PBS). 2. For long term storage, the stock solution can be aliquoted and stored at -20 degree C. For short term storage, the solution can be kept at 2-6 degree C, protected from light. When handled properly, DAPI solutions are stable for at least six months. 3. Dilute the DAPI stock solution to 3 uM in staining buffer. A 1 mL volume will be required for each cell sample. 4. Centrifuge the cell suspension and discard the supernatant. Tap to loosen the pellet and add 1 mL of DAPI diluted in staining buffer. 5. Incubate for 15 minutes at room temperature. 6. Analyze by flow cytometry in the presence of the dye. If the cells are to be viewed by fluorescence microscopy, centrifuge the sample, remove the supernatant and resuspend cells in fresh buffer. Apply a drop of the suspension to a microscope slide, cover with a coverslip and view. For sample preparation protocols for adherent cells and suspension cells, please see the DAPI product datasheet. Researchers should empirically determine the suitability of the DAPI blue fluorescent nuclear DNA dye for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAPI blue fluorescent nuclear DNA dye, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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