Microscopy Imaging
(FIGURE 1: MICROSCOPY IMAGING Fluorescence microscopy imaging of a frozen murine tissue section containing a blood vessel stained with 1 uM Col-F for 30 minutes. Collagen and elastin present in the blood vessel wall stained green. Nuclei were counter-stained blue with DAPI. Image was captured using a Nikon TiE Deconvolution Microscope. Data courtesy of Dr. Mike Olin, University of Minnesota.)
Col-F Collagen Binding Reagent
Col-F Collagen Binding Reagent
Applications
Fluorescence Microscope
Synonyms
Col-F Collagen Binding Reagent; Collagen and Elastin; Col-F Collagen Binding Reagent reagent
Applicable Applications for Col-F Collagen Binding Reagent reagent
Fluorescence Microscope
Application Notes
How to use:
Col-F is supplied as a lyophilized powder. Typical staining concen-trations range between 1-20 μM. However, the optimal staining concentration will vary for diff erent situations and therefore needs to be determined experimentally by the end user. The ideal staining time varies depending on application, and can range from 5-10 minutes to several hours. Optimal staining times or pe-riods will need to be determined experimentally by the user. ICT recommends staining for 30 minutes as a starting point. To stain collagen and elastin:
1. When ready to use, reconstitute the 0.5 mg Col-F vial by adding 100 uL DMSO (this creates a stock at 6.8 mM).
2. Optional: Incubate samples with blocking buffer for 60 minutes.
3. Add Col-F to the media/buffer for each appropriate sample.
4. Incubate the samples with the dye solutions for 5-60 minutes at 37°C.
5. Wash samples twice with PBS (allow samples to incubate at room temp for 5-10 minutes in PBS during each wash step)
6. Once wash steps are complete, mount a coverslip on each slide. Samples are now ready to visualize with a fluorescence microscope capable of excitation at 490 nm, and emission at 515-520 nm.
Col-F is supplied as a lyophilized powder. Typical staining concen-trations range between 1-20 μM. However, the optimal staining concentration will vary for diff erent situations and therefore needs to be determined experimentally by the end user. The ideal staining time varies depending on application, and can range from 5-10 minutes to several hours. Optimal staining times or pe-riods will need to be determined experimentally by the user. ICT recommends staining for 30 minutes as a starting point. To stain collagen and elastin:
1. When ready to use, reconstitute the 0.5 mg Col-F vial by adding 100 uL DMSO (this creates a stock at 6.8 mM).
2. Optional: Incubate samples with blocking buffer for 60 minutes.
3. Add Col-F to the media/buffer for each appropriate sample.
4. Incubate the samples with the dye solutions for 5-60 minutes at 37°C.
5. Wash samples twice with PBS (allow samples to incubate at room temp for 5-10 minutes in PBS during each wash step)
6. Once wash steps are complete, mount a coverslip on each slide. Samples are now ready to visualize with a fluorescence microscope capable of excitation at 490 nm, and emission at 515-520 nm.
Preparation and Storage
Store at ≤ -20°C.
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Microscopy Imaging
(FIGURE 1: MICROSCOPY IMAGING Fluorescence microscopy imaging of a frozen murine tissue section containing a blood vessel stained with 1 uM Col-F for 30 minutes. Collagen and elastin present in the blood vessel wall stained green. Nuclei were counter-stained blue with DAPI. Image was captured using a Nikon TiE Deconvolution Microscope. Data courtesy of Dr. Mike Olin, University of Minnesota.)
Microscopy Imaging
(FIGURE 1: MICROSCOPY IMAGING Fluorescence microscopy imaging of a frozen murine tissue section containing a blood vessel stained with 1 uM Col-F for 30 minutes. Collagen and elastin present in the blood vessel wall stained green. Nuclei were counter-stained blue with DAPI. Image was captured using a Nikon TiE Deconvolution Microscope. Data courtesy of Dr. Mike Olin, University of Minnesota.)
Flourescence Spectra
Flourescence Spectra
Testing Data
Testing Data
Related Product Information for Col-F Collagen Binding Reagent reagent
Collagens and elastins are primarily synthesized by fibroblasts. These molecules are principal components of extracellular matrices. Once outside the cell, collagen assembles into fibrils and fibers that provide mechanical strength to tissues. Elastin is secreted by cells, and also forms fibers which crosslink to create a flexible network of fibers and sheets.
Col-F is a low molecular weight fluorescent probe that exhibits aff inity for collagen and elastin. Col-F easily penetrates between cells and into tissues where it can then bind to collagen and elas-tin fibers via a noncovalent mechanism. Col-F is recommended for staining fresh or frozen tissues. Col-F is not recommended for use with fixed tissues.
Each vial contains 0.5 mg of dry powder Col-F. Once reconstituted in DMSO, Col-F is ready to use: just add it to the sample media, incubate, wash, and analyze. The optimal excitation is 490 nm and emission is 515-520 nm. Col-F is revealed under a microscope using a FITC-compatible filter set. Col-F is for research use only.Not for use in test procedures .
Col-F is a low molecular weight fluorescent probe that exhibits aff inity for collagen and elastin. Col-F easily penetrates between cells and into tissues where it can then bind to collagen and elas-tin fibers via a noncovalent mechanism. Col-F is recommended for staining fresh or frozen tissues. Col-F is not recommended for use with fixed tissues.
Each vial contains 0.5 mg of dry powder Col-F. Once reconstituted in DMSO, Col-F is ready to use: just add it to the sample media, incubate, wash, and analyze. The optimal excitation is 490 nm and emission is 515-520 nm. Col-F is revealed under a microscope using a FITC-compatible filter set. Col-F is for research use only.Not for use in test procedures .
Product Categories/Family for Col-F Collagen Binding Reagent reagent
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Product Notes
The Col-F Collagen Binding Reagent (Catalog #AAA258089) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Col-F Collagen Binding Reagent can be used in a range of immunoassay formats including, but not limited to, Fluorescence Microscope. How to use: Col-F is supplied as a lyophilized powder. Typical staining concen-trations range between 1-20 μM. However, the optimal staining concentration will vary for diff erent situations and therefore needs to be determined experimentally by the end user. The ideal staining time varies depending on application, and can range from 5-10 minutes to several hours. Optimal staining times or pe-riods will need to be determined experimentally by the user. ICT recommends staining for 30 minutes as a starting point. To stain collagen and elastin: 1. When ready to use, reconstitute the 0.5 mg Col-F vial by adding 100 uL DMSO (this creates a stock at 6.8 mM). 2. Optional: Incubate samples with blocking buffer for 60 minutes. 3. Add Col-F to the media/buffer for each appropriate sample. 4. Incubate the samples with the dye solutions for 5-60 minutes at 37°C. 5. Wash samples twice with PBS (allow samples to incubate at room temp for 5-10 minutes in PBS during each wash step) 6. Once wash steps are complete, mount a coverslip on each slide. Samples are now ready to visualize with a fluorescence microscope capable of excitation at 490 nm, and emission at 515-520 nm. Researchers should empirically determine the suitability of the Col-F Collagen Binding Reagent for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Col-F Collagen Binding Reagent, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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