Antibody Pairs Support Pack 2 Reagent

Antibody Pairs Support Pack 2

Cat. Num. AAA2090686

• Synonyms: Antibody Pairs Support Pack 2; Antibody Pairs Support Pack 2 reagent

• Reagents and Materials Provided: Reagents: Coating Buffer; Quantity: 1 bottle of powder; ; Reagents: Blocking Buffer; Quantity: 1 bottle of powder; ; Reagents: Standard Diluent; Quantity: 1 bottle of powder; ; Reagents: Biotin-labeled Competitor Diluent; Quantity: 1 bottle of powder; ; Reagents: Streptavidin-HRP Diluent; Quantity: 1 bottle of powder; ; Reagents: Wash Buffer; Quantity: 1 bottle of powder; ; Reagents: Streptavidin-HRP; Quantity: 1 bottle, 0.6 mL; ; Reagents: Substrate Solution; Quantity: 1 bottle, 48 mL each; ; Reagents: Stop Solution; Quantity: 1 bottle, 30 mL each; ; NOTE: *All of the working solutions should be clear.; *If the working solution is prepared, please store at 2-8ºC.
• Important Notes: Appropriate care should be exercised when using these reagents, including gloves, glasses, lab coats, and good laboratory procedures.
• Applications: Reagents: Coating Buffer; RApplications: Used for coating antibody on the wells of 96-well strip plate. Prepare a working solution by diluting the Coating Buffer with deionized or distilled water before the experiment. 1 bottle can be used for preparing 220mL working solution.; ; Reagents: Blocking Buffer; RApplications: Used for blocking of unspecific binding sites of the plate. Prepare a working solution by diluting the Blocking Buffer with deionized or distilled water before the experiment. 1 bottle can be used for preparing 120mL working solution.; ; Reagents: Standard Diluent; RApplications: Used for dilution of Standard. Prepare a working solution by diluting the Standard Diluent with deionized or distilled water before the experiment. 1 bottle can be used for preparing 80mL working solution.; ; Reagents: Biotinylated Competitor Diluent; RApplications: Used for dilution of Biotinylated Competitor. Prepare a working solution by diluting the Biotin-labeled Competitor Diluent with deionized or distilled water before the experiment. 1 bottle can be used for preparing 60mL working solution.; ; Reagents: Streptavidin-HRP Diluent; RApplications: Used for dilution of Streptavidin-HRP. Prepare a working solution by diluting the Strepavidin-HRP Diluent with deionized or distilled water before the experiment. 1 bottle can be used for preparing 60mL working solution.; ; Reagents: Wash Buffer; RApplications: For plate washing. Prepare a working solution by diluting the Wash Buffer with deionized or distilled water before the experiment. 1 bottle can be used for preparing 2000mL working solution.; ; Reagents: Streptavidin-HRP; RApplications: Strepavidin-HRP can specifically bind biotinylated antigens or antibodies. Prepare a working solution by diluting the Streptavidin-HRP with Streptavidin-HRP Diluent working solution. The dilution ratio is 1:100. 1 bottle can be used for preparing 60mL working solution.; ; Reagents: Substrate Solution; RApplications: Used for chromogenic substrate.; ; Reagents: Stop Solution; RApplications: Used for stopping chromogenic reaction.
• Preparation and Storage: Streptavidin-HRP should be stored at -20 ºC, others are valid for 6 months at 4 ºC. Store diluted (reconstituted powder) or working solutions at 4 ºC for up to one week for use in subsequent experiments.

Related Product Information for Antibody Pairs Support Pack 2 reagent

Antibody Pairs Support Pack is intended to use together with Antibody Pairs for ELISA/CLIA (competitve method) kit preparation. This pack provides reagents for preparation of 5 Competitive ELISA/CLIA plates. All reagents should be used at room temperature and the pack is intended for research purposes only.