Human Pyruvate Dehydrogenase Phosphatase (PDP) RTU ELISA Kit | PDP rtu elisa kit

Human Pyruvate Dehydrogenase Phosphatase (PDP) ELISA Kit

Cat. Num. AAA4501757

• Gene Names: PDP1; PDH; PDP; PDPC; PPM2A; PPM2C
• Reactivity: Human
• Synonyms: Pyruvate Dehydrogenase Phosphatase (PDP); Human Pyruvate Dehydrogenase Phosphatase (PDP) ELISA Kit; PDP1; PDH; PPM2C; Protein Phosphatase 2C Magnesium-Dependent Catalytic Subunit; Pyruvate Dehyrogenase Phosphatase Catalytic Subunit 1; PDP rtu elisa kit

• Reactivity: Human
• Sequence Length: 562

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.099ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDP rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 239985422
• NCBI GeneID: 54704
• NCBI Accession #: NP_001155251.1
• NCBI GenBank Nucleotide #: NM_001161779.1
• UniProt Accession #: Q9P0J1; Q5U5K1; B3KX71; J3KPU0
• Molecular Weight: 63,695 Da
• NCBI Official Full Name: pyruvate dehyrogenase phosphatase catalytic subunit 1 isoform 2
• NCBI Official Synonym Full Names: pyruvate dehyrogenase phosphatase catalytic subunit 1
• NCBI Official Symbol: PDP1
• NCBI Official Synonym Symbols: PDH; PDP; PDPC; PPM2A; PPM2C
• NCBI Protein Information: pyruvate dehyrogenase phosphatase catalytic subunit 1
• UniProt Protein Name: [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
• Protein Family: Petal death protein
• UniProt Gene Name: PDP1
• UniProt Synonym Gene Names: PDP; PPM2C; PDP 1; PDPC 1

NCBI Description

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

Uniprot Description

Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.