Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit | PTGS2 elisa kit

Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit

Cat. Num. AAA2021680

• Gene Names: PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• Reactivity: Rat
• Synonyms: Prostaglandin Endoperoxide Synthase 2 (PTGS2); Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit; PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prostaglandin Endoperoxide Synthase 2 (PTGS2).; No significant cross-reactivity or interference between Prostaglandin Endoperoxide Synthase 2 (PTGS2) and analogues was observed.
• Sequence Length: 604

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTGS2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 2 (PTGS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PTGS2 elisa kit

Enzyme & Kinase; Tumor immunity; Infection immunity; Reproductive science; Pulmonology; Gastroenterology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 1020089
• NCBI GeneID: 5743
• UniProt Accession #: P35354; Q16876; A8K802
• Molecular Weight: 68,996 Da
• NCBI Official Full Name: prostaglandin endoperoxide synthase-2
• NCBI Official Synonym Full Names: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• NCBI Official Symbol: PTGS2
• NCBI Official Synonym Symbols: COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• NCBI Protein Information: prostaglandin G/H synthase 2
• UniProt Protein Name: Prostaglandin G/H synthase 2
• Protein Family: Prostaglandin G/H synthase
• UniProt Gene Name: PTGS2
• UniProt Synonym Gene Names: COX2; COX-2; PGH synthase 2; PGHS-2
• UniProt Entry Name: PGH2_HUMAN

NCBI Description

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Uniprot Description

COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.

Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.14.99.1

Chromosomal Location of Human Ortholog: 1q25.2-q25.3

Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus

Molecular Function: prostaglandin-endoperoxide synthase activity; enzyme binding; protein homodimerization activity; peroxidase activity; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; response to lithium ion; positive regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; decidualization; sensory perception of pain; prostaglandin biosynthetic process; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission, dopaminergic; response to estradiol stimulus; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; regulation of blood pressure; negative regulation of smooth muscle contraction; arachidonic acid metabolic process; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; response to fructose stimulus; water-soluble vitamin metabolic process; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; prostaglandin metabolic process; hair cycle; ovulation; response to manganese ion; NAD metabolic process; regulation of inflammatory response; positive regulation of fever; positive regulation of vasoconstriction; brown fat cell differentiation; response to oxidative stress; cell motility; embryo implantation