Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Peripheral myelin protein 22 (PMP22) Standard Curve.)
Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit | PMP22 elisa kit
Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
Gene Names
Pmp22; Gas-3
Reactivity
Rat
Synonyms
Peripheral Myelin Protein 22 (PMP22); Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit; PMP22; CMT1A; CMT1E; DSS; GAS-3; HMSNIA; HNPP; MGC20769; Sp110; growth arrest-specific 3; PMP22 elisa kit
Reactivity
Rat
Specificity
Rat Peripheral myelin protein 22 (PMP22) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PMP22. No significant cross-reactivity or interference between Rat PMP22 and analogues was observed.
Sequence Length
160
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Peripheral myelin protein 22 (PMP22) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Peripheral myelin protein 22 (PMP22) Standard Curve.)
Related Product Information for PMP22 elisa kit
Description: This Rat Peripheral myelin protein 22 (PMP22) ELISA Kit employs a two-site sandwich ELISA to quantitate PMP22 in samples. An antibody specific for PMP22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PMP22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMP22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMP22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Peripheral myelin protein 22, also known as PMP22, is a protein encoded by the PMP22 gene. Alternative splicing of this gene results in three transcript variants that encode the same protein. The integral membrane protein encoded by this gene is a hydrophobic, tetraspan glycoprotein expressed mainly in Schwann cells and is a major component of compact myelin in the peripheral nervous system.Various mutations of the gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and Hereditary neuropathy with liability to pressure palsies (HNPP).
Background: Peripheral myelin protein 22, also known as PMP22, is a protein encoded by the PMP22 gene. Alternative splicing of this gene results in three transcript variants that encode the same protein. The integral membrane protein encoded by this gene is a hydrophobic, tetraspan glycoprotein expressed mainly in Schwann cells and is a major component of compact myelin in the peripheral nervous system.Various mutations of the gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and Hereditary neuropathy with liability to pressure palsies (HNPP).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Peripheral myelin protein 22
NCBI Official Synonym Full Names
peripheral myelin protein 22
NCBI Official Symbol
Pmp22
NCBI Official Synonym Symbols
Gas-3
NCBI Protein Information
peripheral myelin protein 22
UniProt Protein Name
Peripheral myelin protein 22
Protein Family
UniProt Gene Name
Pmp22
UniProt Synonym Gene Names
Cd25; Pmp-22
UniProt Entry Name
PMP22_RAT
NCBI Description
mediates Schwann cell growth and peripheral myelin compaction; human homolog gene duplication causes Charcot-Marie-Tooth 1A (CMT1A) neuropathy [RGD, Feb 2006]
Research Articles on PMP22
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Product Notes
The Rat PMP22 pmp22 (Catalog #AAA9712104) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712104 ELISA Kit recognizes Rat PMP22. It is sometimes possible for the material contained within the vial of "Peripheral Myelin Protein 22 (PMP22), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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