Rat Fibrinogen Beta (FGb) RTU ELISA Kit | FGb rtu elisa kit

Rat Fibrinogen Beta (FGb) ELISA Kit

Cat. Num. AAA459260

• Gene Names: Fgb; Ab1-181; Ab1-216; Ac1-581
• Reactivity: Rat
• Synonyms: Fibrinogen Beta (FGb); Rat Fibrinogen Beta (FGb) ELISA Kit; FG-B; Fibrinopeptide B; FGb rtu elisa kit

• Reactivity: Rat
• Sequence Length: 479

• Assay Type: Sandwich
• Samples: Plasma.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.65ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGb rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGb. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FGb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FGb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FGb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 158186678
• NCBI GeneID: 24366
• NCBI Accession #: NP_064456.2
• NCBI GenBank Nucleotide #: NM_020071.2
• UniProt Accession #: P14480; Q5I0P7; Q7TME5
• Molecular Weight: 56,613 Da
• NCBI Official Full Name: fibrinogen beta chain
• NCBI Official Synonym Full Names: fibrinogen beta chain
• NCBI Official Symbol: Fgb
• NCBI Official Synonym Symbols: Ab1-181; Ab1-216; Ac1-581
• NCBI Protein Information: fibrinogen beta chain
• UniProt Protein Name: Fibrinogen beta chain
• Protein Family: Fibrinogen
• UniProt Gene Name: Fgb

NCBI Description

cleaved by thrombin; plays a role in wound healing [RGD, Feb 2006]

Uniprot Description

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.